Literature DB >> 1729216

Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolism.

D Ochs1, C Kaletta, K D Entian, A Beck-Sickinger, K Poralla.   

Abstract

The pentacyclic hopanoids, a class of eubacterial lipids, are synthesized by squalene-hopene cyclase and side chain-elongating enzymes. With the aid of DNA probes based on the amino-terminal sequence of purified squalene-hopene cyclase from Bacillus acidocaldarius, clones of Escherichia coli that express this enzyme in the cytoplasmic membrane were isolated. According to the DNA sequence, the cyclase contained 627 amino acids with a molecular mass of 69,473 Da. A high percentage of the amino acids were basic. No significant similarity to existing sequenced proteins was found.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1729216      PMCID： PMC205708          DOI： 10.1128/jb.174.1.298-302.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin: anti-digoxigenin ELISA principle (digoxigenin system).

Authors:  C Kessler; H J Höltke; R Seibl; J Burg; K Mühlegger
Journal:  Biol Chem Hoppe Seyler       Date:  1990-10

2.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

3.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

4.  A comprehensive sequence analysis program for the IBM personal computer.

Authors:  C Queen; L J Korn
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

5.  An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.

Authors:  D Tautz; M Renz
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

6.  Bacteriohopanetetrol: abundant lipid in frankia cells and in nitrogen-fixing nodule tissue.

Authors:  A M Berry; R A Moreau; A D Jones
Journal:  Plant Physiol       Date:  1991-01       Impact factor: 8.340

7.  Cloning and characterization of the 2,3-oxidosqualene cyclase-coding gene of Candida albicans.

Authors:  R Kelly; S M Miller; M H Lai; D R Kirsch
Journal:  Gene       Date:  1990-03-15       Impact factor: 3.688

8.  Interaction of hopanoids with phosphatidylcholines containing oleic and omega-cyclohexyldodecanoic acid in lipid bilayer membranes.

Authors:  R Benz; D Hallmann; K Poralla; H Eibl
Journal:  Chem Phys Lipids       Date:  1983-12       Impact factor: 3.329

9.  The influence of hopanoids on growth of Mycoplasma mycoides.

Authors:  E Kannenberg; K Poralla
Journal:  Arch Microbiol       Date:  1982-12       Impact factor: 2.552

10.  The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.

Authors:  R B Wallace; M J Johnson; T Hirose; T Miyake; E H Kawashima; K Itakura
Journal:  Nucleic Acids Res       Date:  1981-02-25       Impact factor: 16.971
View more
  17 in total

1.  Crystallization and preliminary X-ray crystallographic analysis of squalene-hopene cyclase from Alicyclobacillus acidocaldarius.

Authors:  K U Wendt; C Feil; A Lenhart; K Poralla; G E Schulz
Journal:  Protein Sci       Date:  1997-03       Impact factor: 6.725

2.  Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin.

Authors:  T Dairi; Y Hamano; T Kuzuyama; N Itoh; K Furihata; H Seto
Journal:  J Bacteriol       Date:  2001-10       Impact factor: 3.490

3.  Cloning and characterization of the Dictyostelium discoideum cycloartenol synthase cDNA.

Authors:  S M Godzina; M A Lovato; M M Meyer; K A Foster; W K Wilson; W Gu; E L de Hostos; S P Matsuda
Journal:  Lipids       Date:  2000-03       Impact factor: 1.880

4.  Rationally designed inhibitors as tools for comparing the mechanism of squalene-hopene cyclase with oxidosqualene cyclase.

Authors:  F Viola; M Ceruti; L Cattel; P Milla; K Poralla; G Balliano
Journal:  Lipids       Date:  2000-03       Impact factor: 1.880

5.  Vinyl sulfide derivatives of truncated oxidosqualene as selective inhibitors of oxidosqualene and squalene-hopene cyclases.

Authors:  M Ceruti; G Balliano; F Rocco; P Milla; S Arpicco; L Cattel; F Viola
Journal:  Lipids       Date:  2001-06       Impact factor: 1.880

Review 6.  Squalene-hopene cyclases.

Authors:  Gabriele Siedenburg; Dieter Jendrossek
Journal:  Appl Environ Microbiol       Date:  2011-04-29       Impact factor: 4.792

7.  Conjugated methyl sulfide and phenyl sulfide derivatives of oxidosqualene as inhibitors of oxidosqualene and squalene-hopene cyclases.

Authors:  Flavio Rocco; Simonetta Oliaro Bosso; Franca Viola; Paola Milla; Giorgio Roma; Giancarlo Grossi; Maurizio Ceruti
Journal:  Lipids       Date:  2003-03       Impact factor: 1.880

8.  Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen.

Authors:  E J Corey; S P Matsuda; B Bartel
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-15       Impact factor: 11.205

9.  Isolation and characterization of the gene encoding 2,3-oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae.

Authors:  Z Shi; C J Buntel; J H Griffin
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

10.  Identification of the active site of vertebrate oxidosqualene cyclase.

Authors:  I Abe; G D Prestwich
Journal:  Lipids       Date:  1995-03       Impact factor: 1.880
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.