Effect of occupational stress and anxiety on natural killer lymphocyte activity of men and women employed in a university.

The aim of this study is the immune response of the staff of a university and a museum (referent group). 46 women and 40 men with similar mean age (44 years) were investigated. The first group of women (A) worked in libraries, B in offices in contact with students, C as secretaries and D in a museum. One group of men (E) was composed of employees of the university, one worked in a library temporarily located underground (F) and the last group (G) in a museum. STAI I and II (state and trait anxiety, respectively), and occupational stress were measured by questionnaires. Blood samples were collected for determining NK cytotoxic activity vs human erythroleukaemia cells and the lymphocyte subsets CD45+, CD45+-CD3+, CD45+-CD3+-CD4+, CD45+-CD3+-CD8+, CD45+-CD3-CD8+, CD3+-CD16+-56+ and CD3+-CD19+. Group B showed significantly higher score of occupational stress and STAI I (but not of STAI II) than the other groups of women. Group F of men showed higher levels of occupational stress and both STAI I and II than groups E and G. Group B of women and F of men also showed significantly lower values of blood cytotoxic activity. The scores of STAI I and II were negatively correlated with the cytotoxic activity expressed per ml of blood and/or total lymphocytes. and/or NK CD45+-CD16+-CD56+ cells. In conclusion, this study shows that occupational stress may increase anxiety which is negatively correlated with blood cytotoxic activity. This is a useful tool for determining the health effects of stress.