| Literature DB >> 17289224 |
Małgorzata Kesik1, Luiza Jedlina-Panasiuk, Monika Kozak-Cieszczyk, Andrzej Płucienniczak, Halina Wedrychowicz.
Abstract
Cysteine proteinases released by Fasciola hepatica play a key role in parasite feeding, migration through host tissues and in immune evasion. Hence, a recombinant cysteine proteinase (CPFhW) expressed as inclusion bodies in Escherichia coli was used for enteral vaccination of rats against fasciolosis. We managed to activate this proteinase and found it to have cathepsin L1-like substrate preference. Enteral vaccination of rats induced a 78-80% protection against challenge with fluke metacercariae (mc). The immunised rats showed clear immunological response. The challenge with mc caused a remarkable infiltration of eosinophils into the peritoneal cavity of both the vaccinated rats and challenge control rats. However, CD8+ and CD4+ lymphocytes appeared in significantly higher numbers in the peritoneal fluid of vaccinated rats than in controls.Entities:
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Year: 2007 PMID： 17289224 DOI： 10.1016/j.vaccine.2007.01.057
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641