OBJECTIVE: To determine the prevalence and genotypes of TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae in Guangzhou area. METHODS: Genotype determination of TEM- and SHV-type beta-lactamases were performed by PCR amplification followed by denaturing high-performance liquid chromatography (DHPLC) and DNA sequencing among 57 strain of klebsiella pneumonia obtained from the hospitals in Guangzhou. RESULTS: The genes bla(TEM) and bla(SHV) were amplified from all strains. TEM-116 type ESBLs were found in 56.14% (32/57) of the isolates. SHV-12 (n = 19), -11 (n = 11), -2a (n = 6), -28 (n = 4), -26 (n = 1), -33 (n = 1) and five novel SHV-type beta-lactamases were identified among the SHV-type beta-lactamases. These novel enzymes were designated from SHV-64, -65, -66, -67 and SHV-69, which derived from SHV-1 beta-lactamase or SHV-12 ESBL. A total of 94.74% of the isolates (54/57) produced both TEM- and SHV-type beta-lactamases simultaneously, in which 13.0% were TEM-116 and SHV-12 (n = 12). CONCLUSION: TEM-116 and SHV-12 are the predominant TEM- and SHV-type genotypes of Klebsiella pneumoniae producing beta-lactamases in Guangzhou. This area is confronted with the prevalence of the phenotypes of Klebsiella pneumoniae that simultaneously produce TEM- and SHV-type beta-lactamases.
OBJECTIVE: To determine the prevalence and genotypes of TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae in Guangzhou area. METHODS: Genotype determination of TEM- and SHV-type beta-lactamases were performed by PCR amplification followed by denaturing high-performance liquid chromatography (DHPLC) and DNA sequencing among 57 strain of klebsiella pneumonia obtained from the hospitals in Guangzhou. RESULTS: The genes bla(TEM) and bla(SHV) were amplified from all strains. TEM-116 type ESBLs were found in 56.14% (32/57) of the isolates. SHV-12 (n = 19), -11 (n = 11), -2a (n = 6), -28 (n = 4), -26 (n = 1), -33 (n = 1) and five novel SHV-type beta-lactamases were identified among the SHV-type beta-lactamases. These novel enzymes were designated from SHV-64, -65, -66, -67 and SHV-69, which derived from SHV-1 beta-lactamase or SHV-12 ESBL. A total of 94.74% of the isolates (54/57) produced both TEM- and SHV-type beta-lactamases simultaneously, in which 13.0% were TEM-116 and SHV-12 (n = 12). CONCLUSION: TEM-116 and SHV-12 are the predominant TEM- and SHV-type genotypes of Klebsiella pneumoniae producing beta-lactamases in Guangzhou. This area is confronted with the prevalence of the phenotypes of Klebsiella pneumoniae that simultaneously produce TEM- and SHV-type beta-lactamases.