PURPOSE: ATP causes airway smooth-muscle contraction in patients with asthma and chronic obstructive pulmonary disease. Propofol and ketamine attenuate the airway smooth-muscle contraction induced by histamine and acetylcholine. However, it is not clear whether propofol and ketamine affect the ATP-induced airway smooth-muscle contraction. METHODS: We examined the effects of propofol and ketamine on the ATP-induced contraction and ATP-P(2)-purinoceptor binding. RESULTS: Propofol attenuated the ATP-induced contraction in a dose-dependent manner, with a 50% inhibitory concentration of 54 +/- 22 microM. Ketamine at 300 microM attenuated the ATP-induced contraction. In the binding study, propofol attenuated the binding of the P(2)-purinoceptor with [(3)H]-ATP in a dose-dependent manner, while ketamine did not attenuate this binding. CONCLUSION: Propofol attenuates ATP-induced contraction through the inhibition of ATP-P(2)-purinoceptor binding.
PURPOSE:ATP causes airway smooth-muscle contraction in patients with asthma and chronic obstructive pulmonary disease. Propofol and ketamine attenuate the airway smooth-muscle contraction induced by histamine and acetylcholine. However, it is not clear whether propofol and ketamine affect the ATP-induced airway smooth-muscle contraction. METHODS: We examined the effects of propofol and ketamine on the ATP-induced contraction and ATP-P(2)-purinoceptor binding. RESULTS:Propofol attenuated the ATP-induced contraction in a dose-dependent manner, with a 50% inhibitory concentration of 54 +/- 22 microM. Ketamine at 300 microM attenuated the ATP-induced contraction. In the binding study, propofol attenuated the binding of the P(2)-purinoceptor with [(3)H]-ATP in a dose-dependent manner, while ketamine did not attenuate this binding. CONCLUSION:Propofol attenuates ATP-induced contraction through the inhibition of ATP-P(2)-purinoceptor binding.
Authors: S Shibata; M Niwa; A Himeno; N G Gana; K Shigematsu; M Matsumoto; K Yamashita; K Sumikawa; K Taniyama Journal: Cell Mol Neurobiol Date: 1997-02 Impact factor: 5.046