A Shakoor1, M Gupta, N P Blair, M Shahidi. 1. Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.
Abstract
PURPOSE: To establish baseline and variability of oxygen tension (PO(2)) measurements in the choroid, retinal arteries, capillaries, and veins of spontaneously breathing anesthetized rats and determine the effect of a moderate surgical procedure on the chorioretinal PO(2). METHODS: Our previously established optical section phosphorescence imaging technique was utilized to measure PO(2) in the chorioretinal vasculatures. Imaging was performed in 29 spontaneously breathing rats under ketamine/xylazine anesthesia. In 7 rats, blood was drawn using a surgically implanted femoral arterial catheter and analyzed to determine the systemic arterial PO(2). The PO(2) measurements in 22 rats without surgery (group 1) and 7 surgically instrumented rats (group 2) were statistically compared. The intrasubject variability was calculated by the average standard deviation (SD) of repeated measurements. RESULTS: The average systemic arterial PO(2) was 52 +/- 7 mm Hg (mean +/- SD) in group 2. In group 1, the average PO(2) measurements in the choroid, retinal arteries, capillaries, and veins were 50 +/- 11, 40 +/- 5, 39 +/- 6, and 30 +/- 5 mm Hg, respectively. No statistically significant PO(2) differences in any of the chorioretinal vasculatures were found between the two groups (p > 0.4). The intrasubject variability was 3 mm Hg in the choroid, retinal arteries, capillaries, and veins. CONCLUSIONS: Chorioretinal PO(2) measurements in spontaneously breathing anesthetized rats have a relatively low variability, indicating that PO(2) changes due to various physiological alterations can be reliably assessed.
PURPOSE: To establish baseline and variability of oxygen tension (PO(2)) measurements in the choroid, retinal arteries, capillaries, and veins of spontaneously breathing anesthetized rats and determine the effect of a moderate surgical procedure on the chorioretinal PO(2). METHODS: Our previously established optical section phosphorescence imaging technique was utilized to measure PO(2) in the chorioretinal vasculatures. Imaging was performed in 29 spontaneously breathing rats under ketamine/xylazine anesthesia. In 7 rats, blood was drawn using a surgically implanted femoral arterial catheter and analyzed to determine the systemic arterial PO(2). The PO(2) measurements in 22 rats without surgery (group 1) and 7 surgically instrumented rats (group 2) were statistically compared. The intrasubject variability was calculated by the average standard deviation (SD) of repeated measurements. RESULTS: The average systemic arterial PO(2) was 52 +/- 7 mm Hg (mean +/- SD) in group 2. In group 1, the average PO(2) measurements in the choroid, retinal arteries, capillaries, and veins were 50 +/- 11, 40 +/- 5, 39 +/- 6, and 30 +/- 5 mm Hg, respectively. No statistically significant PO(2) differences in any of the chorioretinal vasculatures were found between the two groups (p > 0.4). The intrasubject variability was 3 mm Hg in the choroid, retinal arteries, capillaries, and veins. CONCLUSIONS: Chorioretinal PO(2) measurements in spontaneously breathing anesthetized rats have a relatively low variability, indicating that PO(2) changes due to various physiological alterations can be reliably assessed.
Authors: Bruce A Berkowitz; Robin Roberts; Hongmei Luan; Jamie Peysakhov; Deborah L Knoerzer; Jane R Connor; Thomas C Hohman Journal: Invest Ophthalmol Vis Sci Date: 2005-08 Impact factor: 4.799
Authors: David F Wilson; Sergei A Vinogradov; Pavel Grosul; M Noel Vaccarezza; Akiko Kuroki; Jean Bennett Journal: Appl Opt Date: 2005-09-01 Impact factor: 1.980
Authors: R A Linsenmeier; R D Braun; M A McRipley; L B Padnick; J Ahmed; D L Hatchell; D S McLeod; G A Lutty Journal: Invest Ophthalmol Vis Sci Date: 1998-08 Impact factor: 4.799