| Literature DB >> 17280752 |
Silvia Massa1, Rosella Franconi, Rossella Brandi, Antonio Muller, Vadim Mett, Vidadi Yusibov, Aldo Venuti.
Abstract
The E7 oncoprotein from Human Papilloma Virus (HPV) is an attractive candidate for anti-cancer vaccine development. In this study, we engineered HPV16 E7 coding sequence (wild type or mutagenized sequence, E7GGG) as fusions to beta-1,3-1,4-glucanase (LicKM) of Clostridium thermocellum and produced in Nicotiana benthamiana plants using a transient expression system. Target antigens were purified and evaluated in mice for their potential as prophylactic and therapeutic vaccine candidates. Both fusion proteins induced E7-specific IgG and cytotoxic T-cell responses and protected mice against challenge with E7-expressing tumor cells. Furthermore, when administered after challenge, these plant-produced antigens prevented tumor development.Entities:
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Year: 2007 PMID： 17280752 DOI： 10.1016/j.vaccine.2007.01.018
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641