Literature DB >> 17279394

Discrimination of Cryptosporidium species by denaturing gradient gel electrophoresis.

Masaaki Satoh1, Yutaka Nakai.   

Abstract

Denaturing gradient gel electrophoresis (DGGE) was used for the discrimination of three species and one genotype of the protozoan parasite Cryptosporidium: the C. parvum, C. andersoni, C. muris, and C. muris Japanese field mouse genotype. A set of primers specific for the 18S rRNA gene of Cryptosporidium was used in the DGGE; consequently, the four strains showed different banding patterns. This is a potentially convenient and precise method for the discrimination of Cryptosporidium spp.

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Year:  2007        PMID： 17279394     DOI： 10.1007/s00436-007-0466-2

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  21 in total

1.  The identification and characterisation of an unusual genotype of Cryptosporidium from human faeces as Cryptosporidium meleagridis.

Authors:  S Pedraza-Díaz; C Amar; J McLauchlin
Journal:  FEMS Microbiol Lett       Date:  2000-08-15       Impact factor: 2.742

2.  Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health.

Authors:  Lihua Xiao; Irshad M Sulaiman; Una M Ryan; Ling Zhou; Edward R Atwill; Monica L Tischler; Xichen Zhang; Ronald Fayer; Altaf A Lal
Journal:  Int J Parasitol       Date:  2002-12-19       Impact factor: 3.981

3.  Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites.

Authors:  L Xiao; J Limor; U M Morgan; I M Sulaiman; R C Thompson; A A Lal
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

4.  A novel genotype of Cryptosporidium muris from large Japanese field mice, Apodemus speciosus.

Authors:  Kenji Hikosaka; Yutaka Nakai
Journal:  Parasitol Res       Date:  2005-09-07       Impact factor: 2.289

5.  Evaluation of Cryptosporidium parvum genotyping techniques.

Authors:  I M Sulaiman; L Xiao; A A Lal
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

6.  [Cryptosporidium diarrhea developing in two Japanese adults--one in AIDS and the other in a normal host. Research Group for Infectious Enteric Diseases, Japan].

Authors:  G Masuda; M Negishi; A Ajisawa; T Yamaguchi; T Tajima; S Tamagawa; Y Maeda; H Ohtomo; I Kimata; S Uni
Journal:  Kansenshogaku Zasshi       Date:  1991-12

7.  Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa).

Authors:  U M Ryan; P Monis; H L Enemark; I Sulaiman; B Samarasinghe; C Read; R Buddle; I Robertson; L Zhou; R C A Thompson; L Xiao
Journal:  J Parasitol       Date:  2004-08       Impact factor: 1.276

8.  Detection of Cryptosporidium muris type oocysts from beef cattle in a farm and from domestic and wild animals in and around the farm.

Authors:  Yutaka Nakai; Kenji Hikosaka; Masaaki Sato; Takako Sasaki; Yoshiyasu Kaneta; Noriyuki Okazaki
Journal:  J Vet Med Sci       Date:  2004-08       Impact factor: 1.267

9.  Denaturing gradient gel electrophoresis: a rapid method for differentiating BoLA-DRB3 alleles.

Authors:  B M Aldridge; S M McGuirk; R J Clark; L A Knapp; D I Watkins; D P Lunn
Journal:  Anim Genet       Date:  1998-10       Impact factor: 3.169

Review 10.  Genomics and genetics of Cryptosporidium parvum: the key to understanding cryptosporidiosis.

Authors:  Giovanni Widmer; Lunhui Lin; Vivek Kapur; Xiaochuan Feng; Mitchell S Abrahamsen
Journal:  Microbes Infect       Date:  2002-08       Impact factor: 2.700
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