Literature DB >> 17277453

Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae.

Wei Liu1, Ye-Yun Zhou, Mai-Kun Teng, Cong-Zhao Zhou.   

Abstract

The heat-shock protein Hsp33 from the yeast Saccharomyces cerevisiae has been overexpressed, purified and crystallized. A crystal was obtained using the hanging-drop vapour-diffusion method and a data set was collected to 2.7 A resolution. The crystal belongs to space group P4(3)2(1)2, with unit-cell parameters a = b = 96.43, c = 132.22 A, alpha = beta = gamma = 90 degrees . The asymmetric unit is assumed to contain two subunits of Hsp33, with a V(M) value of 2.96 A(3) Da(-1) and a solvent content of 58.41%.

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Year:  2007        PMID： 17277453      PMCID： PMC2330124          DOI： 10.1107/S1744309107000681

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  The 1.6-A crystal structure of the class of chaperones represented by Escherichia coli Hsp31 reveals a putative catalytic triad.

Authors:  Paulene M Quigley; Konstantin Korotkov; Francois Baneyx; Wim G J Hol
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-05       Impact factor: 11.205

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Authors:  Abderrahim Malki; Thérèse Caldas; Jad Abdallah; Renée Kern; Viola Eckey; So Jung Kim; Sun-Shin Cha; Hirotada Mori; Gilbert Richarme
Journal:  J Biol Chem       Date:  2004-11-18       Impact factor: 5.157

6.  Crystal structure of the YDR533c S. cerevisiae protein, a class II member of the Hsp31 family.

Authors:  Marc Graille; Sophie Quevillon-Cheruel; Nicolas Leulliot; Cong-Zhao Zhou; Ines Li de la Sierra Gallay; Lilian Jacquamet; Jean-Luc Ferrer; Dominique Liger; Anne Poupon; Joel Janin; Herman van Tilbeurgh
Journal:  Structure       Date:  2004-05       Impact factor: 5.006

7.  Systematic analysis of HSP gene expression and effects on cell growth and survival at high hydrostatic pressure in Saccharomyces cerevisiae.

Authors:  Takeshi Miura; Hiroaki Minegishi; Ron Usami; Fumiyoshi Abe
Journal:  Extremophiles       Date:  2006-02-18       Impact factor: 2.395

8.  The 1.8-A resolution crystal structure of YDR533Cp from Saccharomyces cerevisiae: a member of the DJ-1/ThiJ/PfpI superfamily.

Authors:  Mark A Wilson; Courtney V St Amour; Jennifer L Collins; Dagmar Ringe; Gregory A Petsko
Journal:  Proc Natl Acad Sci U S A       Date:  2004-01-26       Impact factor: 11.205

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Journal:  Microbiol Rev       Date:  1995-09
  9 in total
  1 in total

1.  Structure of Hsp33/YOR391Cp from the yeast Saccharomyces cerevisiae.

Authors:  Peng Chao Guo; Ye Yun Zhou; Xiao Xiao Ma; Wei Fang Li
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-11-16
  1 in total

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