Literature DB >> 17277450

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus.

Shankar Prasad Kanaujia1, Chellamuthu Vasuki Ranjani, Jeyaraman Jeyakanthan, Seiki Baba, Chizu Kuroishi, Akio Ebihara, Akeo Shinkai, Seiki Kuramitsu, Yoshitsugu Shiro, Kanagaraj Sekar, Shigeyuki Yokoyama.   

Abstract

The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K(2)) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C222(1), with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 A. The crystal diffracted to a resolution of 2.2 A. Preliminary studies and molecular-replacement calculations reveal the presence of three monomers in the asymmetric unit.

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Year:  2007        PMID: 17277450      PMCID: PMC2330136          DOI: 10.1107/S1744309106056521

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  12 in total

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Journal:  Nature       Date:  1992-01-30       Impact factor: 49.962

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Authors:  H Takami; A Inoue; F Fuji; K Horikoshi
Journal:  FEMS Microbiol Lett       Date:  1997-07-15       Impact factor: 2.742

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5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

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Authors:  S Kuramitsu; K Hiromi; H Hayashi; Y Morino; H Kagamiyama
Journal:  Biochemistry       Date:  1990-06-12       Impact factor: 3.162

7.  Menaquinone (vitamin K2) biosynthesis: nucleotide sequence and expression of the menB gene from Escherichia coli.

Authors:  V Sharma; K Suvarna; R Meganathan; M E Hudspeth
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

8.  Biosynthesis of o-succinylbenzoic acid in a men- Escherichia coli mutant requires decarboxylation of L-glutamate at the C-1 position.

Authors:  R Meganathan; R Bentley
Journal:  Biochemistry       Date:  1981-09-01       Impact factor: 3.162

9.  Crystal structure of Mycobacterium tuberculosis MenB, a key enzyme in vitamin K2 biosynthesis.

Authors:  James J Truglio; Karsten Theis; Yuguo Feng; Ramona Gajda; Carl Machutta; Peter J Tonge; Caroline Kisker
Journal:  J Biol Chem       Date:  2003-08-08       Impact factor: 5.157

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Authors:  Hideto Takami; Shinro Nishi; Jei Lu; Shigeru Shimamura; Yoshihiro Takaki
Journal:  Extremophiles       Date:  2004-05-27       Impact factor: 2.395

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  2 in total

1.  Structural basis of the induced-fit mechanism of 1,4-dihydroxy-2-naphthoyl coenzyme A synthase from the crotonase fold superfamily.

Authors:  Yueru Sun; Haigang Song; Jie Li; Yan Li; Ming Jiang; Jiahai Zhou; Zhihong Guo
Journal:  PLoS One       Date:  2013-04-26       Impact factor: 3.240

2.  Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR).

Authors:  Kenji Fukui; Yoshitaka Bessho; Atsuhiro Shimada; Shigeyuki Yokoyama; Seiki Kuramitsu
Journal:  Int J Mol Sci       Date:  2013-03-21       Impact factor: 5.923

  2 in total

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