Literature DB >> 17277442

Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions.

Hiroaki Tanaka1, Takashi Umehara, Koji Inaka, Sachiko Takahashi, Rie Shibata, Yoshitaka Bessho, Masaru Sato, Shigeru Sugiyama, Emiko Fusatomi, Takaho Terada, Mikako Shirouzu, Satoshi Sano, Moritoshi Motohara, Tomoyuki Kobayashi, Tetsuo Tanaka, Akiko Tanaka, Shigeyuki Yokoyama.   

Abstract

The transcription termination factor NusA from Aeropyrum pernix was crystallized using a counter-diffusion technique in both terrestrial and microgravity environments. Crystallization under microgravity conditions significantly reduced the twinning content (1.0%) compared with terrestrially grown crystals (18.3%) and improved the maximum resolution from 3.0 to 2.29 A, with similar unit-cell parameters. Based on a comparison of the crystal parameters, the effect of microgravity on protein crystallization is discussed.

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Year:  2007        PMID： 17277442      PMCID： PMC2330117          DOI： 10.1107/S1744309106054625

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  12 in total

1.  An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA.

Authors:  M Worbs; G P Bourenkov; H D Bartunik; R Huber; M C Wahl
Journal:  Mol Cell       Date:  2001-06       Impact factor: 17.970

2.  Crystal structure of the transcription elongation/anti-termination factor NusA from Mycobacterium tuberculosis at 1.7 A resolution.

Authors:  B Gopal; L F Haire; S J Gamblin; E J Dodson; A N Lane; K G Papavinasasundaram; M J Colston; G Dodson
Journal:  J Mol Biol       Date:  2001-12-14       Impact factor: 5.469

3.  A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions.

Authors:  Hiroaki Tanaka; Koji Inaka; Shigeru Sugiyama; Sachiko Takahashi; Satoshi Sano; Masaru Sato; Susumu Yoshitomi
Journal:  J Synchrotron Radiat       Date:  2003-11-28       Impact factor: 2.616

4.  Evidence that the KH RNA-binding domains influence the action of the E. coli NusA protein.

Authors:  Ying Zhou; Thien-Fah Mah; Jack Greenblatt; David I Friedman
Journal:  J Mol Biol       Date:  2002-05-17       Impact factor: 5.469

5.  Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques.

Authors:  Juan Ma Garcia-Ruíz; Luis Antonio Gonzalez-Ramirez; José Antonio Gavira; Fermín Otálora
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-09-26

Review 6.  Bacterial transcription elongation factors: new insights into molecular mechanism of action.

Authors:  Sergei Borukhov; Jookyung Lee; Oleg Laptenko
Journal:  Mol Microbiol       Date:  2005-03       Impact factor: 3.501

7.  Numerical analysis of the depletion zone formation around a growing protein crystal.

Authors:  Hiroaki Tanaka; Koji Inaka; Shigeru Sugiyama; Sachiko Takahashi; Satoshi Sano; Masaru Sato; Susumu Yoshitomi
Journal:  Ann N Y Acad Sci       Date:  2004-11       Impact factor: 5.691

8.  Crystallography & NMR system: A new software suite for macromolecular structure determination.

Authors:  A T Brünger; P D Adams; G M Clore; W L DeLano; P Gros; R W Grosse-Kunstleve; J S Jiang; J Kuszewski; M Nilges; N S Pannu; R J Read; L M Rice; T Simonson; G L Warren
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1998-09-01

Review 9.  Crystal growth and crystallography.

Authors:  A A Chernov
Journal:  Acta Crystallogr A       Date:  1998-11-01       Impact factor: 2.290

10.  Detecting and overcoming crystal twinning.

Authors:  T O Yeates
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600
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  4 in total

1.  High-quality crystals of human haematopoietic prostaglandin D synthase with novel inhibitors.

Authors:  Sachiko Takahashi; Toshiharu Tsurumura; Kosuke Aritake; Naoki Furubayashi; Masaru Sato; Mari Yamanaka; Erika Hirota; Satoshi Sano; Tomoyuki Kobayashi; Tetsuo Tanaka; Koji Inaka; Hiroaki Tanaka; Yoshihiro Urade
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-06-24

2.  Improvement in the quality of hematopoietic prostaglandin D synthase crystals in a microgravity environment.

Authors:  Hiroaki Tanaka; Toshiharu Tsurumura; Kosuke Aritake; Naoki Furubayashi; Sachiko Takahashi; Mari Yamanaka; Erika Hirota; Satoshi Sano; Masaru Sato; Tomoyuki Kobayashi; Tetsuo Tanaka; Koji Inaka; Yoshihiro Urade
Journal:  J Synchrotron Radiat       Date:  2010-11-05       Impact factor: 2.616

3.  High-Quality Protein Crystal Growth of Mouse Lipocalin-Type Prostaglandin D Synthase in Microgravity.

Authors:  Koji Inaka; Sachiko Takahashi; Kosuke Aritake; Toshiharu Tsurumura; Naoki Furubayashi; Bin Yan; Erika Hirota; Satoshi Sano; Masaru Sato; Tomoyuki Kobayashi; Yoshinori Yoshimura; Hiroaki Tanaka; Yoshihiro Urade
Journal:  Cryst Growth Des       Date:  2011-04-05       Impact factor: 4.076

4.  Numerical model of protein crystal growth in a diffusive field such as the microgravity environment.

Authors:  Hiroaki Tanaka; Susumu Sasaki; Sachiko Takahashi; Koji Inaka; Yoshio Wada; Mitsugu Yamada; Kazunori Ohta; Hiroshi Miyoshi; Tomoyuki Kobayashi; Shigeki Kamigaichi
Journal:  J Synchrotron Radiat       Date:  2013-10-01       Impact factor: 2.616
  4 in total

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