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Literature DB >> 17277214

Comparison of a reporter assay and immunomagnetic separation real-time reverse transcription-PCR for the detection of enteroviruses in seeded environmental water samples.

Yu-Chen Hwang¹, Oymon M Leong, Wilfred Chen, Marylynn V Yates.

Abstract

Two newly developed protocols for infective virus detection were compared to the plaque assay. An immunomagnetic separation procedure coupled with real-time reverse transcription-PCR of viral nucleic acids was developed to identify intact enteroviral particles, and a reporter cell system responding to viral replication based on fluorescent resonance energy transfer for detection of infectious enteroviruses was tested. Both new procedures detected infective viruses in environmental samples at the same level as the plaque assay.

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Year: 2007 PMID： 17277214 PMCID： PMC1855679 DOI： 10.1128/AEM.01758-06

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

11 in total

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Journal: Appl Environ Microbiol Date: 2005-06 Impact factor: 4.792

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Authors: Yu-Chen Hwang; Wilfred Chen; Marylynn V Yates
Journal: Appl Environ Microbiol Date: 2006-05 Impact factor: 4.792

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Journal: Nat Biotechnol Date: 1996-03 Impact factor: 54.908

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Authors: Khaled H Abd El Galil; M A El Sokkary; S M Kheira; Andre M Salazar; Marylynn V Yates; Wilfred Chen; Ashok Mulchandani
Journal: Appl Environ Microbiol Date: 2004-07 Impact factor: 4.792

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7 in total

1. Development of an immunomagnetic bead separation-coupled quantitative PCR method for rapid and sensitive detection of Cryptosporidium parvum oocysts in calf feces.

Authors: Shanshan Gao; Min Zhang; Said Amer; Jing Luo; Chengmin Wang; Shaoqiang Wu; Baohua Zhao; Hongxuan He
Journal: Parasitol Res Date: 2014-04-01 Impact factor: 2.289

Comparison of a reporter assay and immunomagnetic separation real-time reverse transcription-PCR for the detection of enteroviruses in seeded environmental water samples.

1. Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection.

2. Survival of infectious Poliovirus-1 in river water compared to the persistence of somatic coliphages, thermotolerant coliforms and Poliovirus-1 genome.

3. Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41.

4. Use of fluorescence resonance energy transfer for rapid detection of enteroviral infection in vivo.

5. Molecular beacons: probes that fluoresce upon hybridization.

6. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR.

7. Immunomagnetic separation of a Norwalk-like virus (genogroup I) in artificially contaminated environmental water samples.

8. Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples.

9. Development and evaluation of a 'real-time' RT-PCR for the detection of enterovirus and parechovirus RNA in CSF and throat swab samples.

Review 10. Magnetic separation techniques in diagnostic microbiology.

1. Development of an immunomagnetic bead separation-coupled quantitative PCR method for rapid and sensitive detection of Cryptosporidium parvum oocysts in calf feces.

2. Detection of adenoviruses and rotaviruses in drinking water sources used in rural areas of Benin, West Africa.

3. Culture-independent evaluation of nonenveloped-virus infectivity reduced by free-chlorine disinfection.

4. Comparison of Two Concentration Methods for the Molecular Detection of Enteroviruses in Raw and Treated Sewage.

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