Literature DB >> 17276678

Degradation of DNA by bisulfite treatment.

Kazuo Tanaka1, Akimitsu Okamoto.   

Abstract

A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.

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Year:  2007        PMID： 17276678     DOI： 10.1016/j.bmcl.2007.01.040

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  55 in total

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