BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.
BACKGROUND:HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.
Authors: A L Feldman; N G Costouros; E Wang; M Qian; F M Marincola; H R Alexander; S K Libutti Journal: Biotechniques Date: 2002-10 Impact factor: 1.993
Authors: Concepcion Conejero-Goldberg; Ena Wang; Chuli Yi; Terry E Goldberg; Lorraine Jones-Brando; Francesco M Marincola; Maree J Webster; E Fuller Torrey Journal: Biotechniques Date: 2005-11 Impact factor: 1.993
Authors: Claudio Cermelli; Rossana Berti; Samantha S Soldan; Michael Mayne; James M D'ambrosia; Samuel K Ludwin; Steven Jacobson Journal: J Infect Dis Date: 2003-04-09 Impact factor: 5.226
Authors: D Donati; N Akhyani; A Fogdell-Hahn; C Cermelli; R Cassiani-Ingoni; A Vortmeyer; J D Heiss; P Cogen; W D Gaillard; S Sato; W H Theodore; S Jacobson Journal: Neurology Date: 2003-11-25 Impact factor: 9.910