OBJECTIVE: Recent work has suggested that neuropilin-1 (NRP-1) is a surface marker of regulatory T cells (Treg). However, no further relative evidence has been provided to confirm this finding. Since Treg should decline during rejection, the expression of NRP-1 on lymphocytes should decline in a rejected graft. To test this proposal, we examined NRP-1 expression in kidney graft biopsies. MATERIALS AND METHODS: Tissue samples were obtained from 20 kidney graft biopsies with pathologically confirmed acute rejection and from 10 without rejections. We performed immunohistochemistry assays using an anti-NRP-1 monoclonal antibody. The positive cells were counted and the ratios among lymphocytes analyzed. RESULTS: Compared with samples from nonrejected graft biopsies (18.71 +/- 20.60), the number of positive cells among lymphocytes in the rejected samples showed a lower percentage (3.16 +/- 1.72; P < .05). CONCLUSIONS: NRP-1 has an important role in directing the growth of nervous synapses and immune synapses. We found in rejected grafts that the percentage of NRP-1 positive cells among lymphocytes decreased significantly. Therefore, NRP-1 may have a previously unrecognized role to predict the immune state of the graft as a potential marker for Treg.
OBJECTIVE: Recent work has suggested that neuropilin-1 (NRP-1) is a surface marker of regulatory T cells (Treg). However, no further relative evidence has been provided to confirm this finding. Since Treg should decline during rejection, the expression of NRP-1 on lymphocytes should decline in a rejected graft. To test this proposal, we examined NRP-1 expression in kidney graft biopsies. MATERIALS AND METHODS: Tissue samples were obtained from 20 kidney graft biopsies with pathologically confirmed acute rejection and from 10 without rejections. We performed immunohistochemistry assays using an anti-NRP-1 monoclonal antibody. The positive cells were counted and the ratios among lymphocytes analyzed. RESULTS: Compared with samples from nonrejected graft biopsies (18.71 +/- 20.60), the number of positive cells among lymphocytes in the rejected samples showed a lower percentage (3.16 +/- 1.72; P < .05). CONCLUSIONS:NRP-1 has an important role in directing the growth of nervous synapses and immune synapses. We found in rejected grafts that the percentage of NRP-1 positive cells among lymphocytes decreased significantly. Therefore, NRP-1 may have a previously unrecognized role to predict the immune state of the graft as a potential marker for Treg.
Authors: Mauricio Campos-Mora; Rodrigo A Morales; Francisco Pérez; Tania Gajardo; Javier Campos; Diego Catalan; Juan Carlos Aguillón; Karina Pino-Lagos Journal: Immunol Cell Biol Date: 2014-09-23 Impact factor: 5.126
Authors: Sohini Roy; Arup K Bag; Rakesh K Singh; James E Talmadge; Surinder K Batra; Kaustubh Datta Journal: Front Immunol Date: 2017-10-10 Impact factor: 7.561