| Literature DB >> 1727426 |
M E Rüffer-Turner1, D J Read, M K Johnson.
Abstract
Neuropathy target esterase from hen brains was radiolabelled at the active site with [3H]diisopropyl phosphorofluoridate. The labelled protein was purified by differential centrifugation and Nonidet P40 solubilization, detergent phase partitioning, anion exchange, and preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The volatilizable counts assay and analytical SDS-PAGE were used to monitor the protein. The 150-kDa subunit polypeptide appears as a single band on analytical SDS-PAGE.Entities:
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Year: 1992 PMID： 1727426 DOI： 10.1111/j.1471-4159.1992.tb09288.x
Source DB: PubMed Journal: J Neurochem ISSN： 0022-3042 Impact factor: 5.372