BACKGROUND: Platelet-derived growth factor (PDGF) has been shown to promote dermal wound healing, however, the molecular mechanisms responsible for are not fully understood. OBJECTIVE: The present study was undertaken to investigate the possible signaling mechanisms by which PDGF improved healing of cutaneous wound in diabetic rats. METHODS: Four full-thickness skin wounds were created on the dorsum of Wistar diabetic rats. Animals were treated with or without recombinant human PDGF (rhPDGF) at 7.0 microg/cm(2) wound or vehicle daily 1 day after wounding. The animals were then killed after various intervals of wounding, and the wounded skin tissues were used for histological evaluation, analysis of the phosphorylation of extracellular signal-regulated kinases (ERK) and the expression of c-fos protein, as well as the labeling indices of proliferative cell nuclear antigen (PCNA). RESULTS: Topical application of rhPDGF significantly accelerated the rate of reepithelialization compared with vehicle-treated or untreated group at 7 days after wounding. At the histological level, the significant increases in the degree of reepithelialization, the thickness of granulation tissue and the density of capillary bud were observed in the wound sites in rhPDGF-treated group at 7 and 14 days after wounding. Moreover, treatment with rhPDGF increased PCNA labeling indices, c-fos protein expression and ERK phosphorylation in the wounded tissues at the indicated time after wounding. CONCLUSION: These results suggest that application of rhPDGF increases cell proliferation, and enhances dermal tissue repair in diabetic skin lesion of rats, which might be partly mediated by ERK activation and c-fos protein expression.
BACKGROUND: Platelet-derived growth factor (PDGF) has been shown to promote dermal wound healing, however, the molecular mechanisms responsible for are not fully understood. OBJECTIVE: The present study was undertaken to investigate the possible signaling mechanisms by which PDGF improved healing of cutaneous wound in diabeticrats. METHODS: Four full-thickness skin wounds were created on the dorsum of Wistar diabeticrats. Animals were treated with or without recombinant human PDGF (rhPDGF) at 7.0 microg/cm(2) wound or vehicle daily 1 day after wounding. The animals were then killed after various intervals of wounding, and the wounded skin tissues were used for histological evaluation, analysis of the phosphorylation of extracellular signal-regulated kinases (ERK) and the expression of c-fos protein, as well as the labeling indices of proliferative cell nuclear antigen (PCNA). RESULTS: Topical application of rhPDGF significantly accelerated the rate of reepithelialization compared with vehicle-treated or untreated group at 7 days after wounding. At the histological level, the significant increases in the degree of reepithelialization, the thickness of granulation tissue and the density of capillary bud were observed in the wound sites in rhPDGF-treated group at 7 and 14 days after wounding. Moreover, treatment with rhPDGF increased PCNA labeling indices, c-fos protein expression and ERK phosphorylation in the wounded tissues at the indicated time after wounding. CONCLUSION: These results suggest that application of rhPDGF increases cell proliferation, and enhances dermal tissue repair in diabetic skin lesion of rats, which might be partly mediated by ERK activation and c-fos protein expression.
Authors: Santosh Gowda; David A Weinstein; Timothy D Blalock; Kavita Gandhi; Bruce A Mast; Gloria Chin; Gregory S Schultz Journal: Int Wound J Date: 2013-10-07 Impact factor: 3.315
Authors: Milena Deptuła; Przemysław Karpowicz; Anna Wardowska; Piotr Sass; Paweł Sosnowski; Alina Mieczkowska; Natalia Filipowicz; Maria Dzierżyńska; Justyna Sawicka; Ewa Nowicka; Paulina Langa; Adriana Schumacher; Mirosława Cichorek; Jacek Zieliński; Karolina Kondej; Franciszek Kasprzykowski; Artur Czupryn; Łukasz Janus; Piotr Mucha; Piotr Skowron; Arkadiusz Piotrowski; Paweł Sachadyn; Sylwia Rodziewicz-Motowidło; Michał Pikuła Journal: Adv Wound Care (New Rochelle) Date: 2019-10-29 Impact factor: 4.730