Literature DB >> 17266655

Large scale preparation of the mammalian high mobility group protein A2 for biophysical studies.

Tengjiao Cui1, Suzanne Joynt, Victor Morillo, Maria Baez, Zhichun Hua, Xiaotang Wang, Fenfei Leng.   

Abstract

Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17266655     DOI： 10.2174/092986607779117281

Source DB:  PubMed          Journal:  Protein Pept Lett        ISSN： 0929-8665            Impact factor:   1.890


  7 in total

1.  Inhibition of high-mobility-group A2 protein binding to DNA by netropsin: a biosensor-surface plasmon resonance assay.

Authors:  Yi Miao; Tengjiao Cui; Fenfei Leng; W David Wilson
Journal:  Anal Biochem       Date:  2007-10-23       Impact factor: 3.365

2.  Binding the mammalian high mobility group protein AT-hook 2 to AT-rich deoxyoligonucleotides: enthalpy-entropy compensation.

Authors:  Suzanne Joynt; Victor Morillo; Fenfei Leng
Journal:  Biophys J       Date:  2009-05-20       Impact factor: 4.033

3.  A rapid and sensitive high-throughput screening method to identify compounds targeting protein-nucleic acids interactions.

Authors:  Nicole Alonso; Roboan Guillen; Jeremy W Chambers; Fenfei Leng
Journal:  Nucleic Acids Res       Date:  2015-02-04       Impact factor: 16.971

4.  AT-hook peptides bind the major and minor groove of AT-rich DNA duplexes.

Authors:  Alyssa Garabedian; Kevin Jeanne Dit Fouque; Prem P Chapagain; Fenfei Leng; Francisco Fernandez-Lima
Journal:  Nucleic Acids Res       Date:  2022-03-21       Impact factor: 19.160

5.  DNA bending by the mammalian high-mobility group protein AT hook 2.

Authors:  Bo Chen; Jasmine Young; Fenfei Leng
Journal:  Biochemistry       Date:  2010-03-02       Impact factor: 3.162

6.  The Dimerization State of the Mammalian High Mobility Group Protein AT-Hook 2 (HMGA2).

Authors:  Lorraine Frost; Maria A M Baez; Christopher Harrilal; Alyssa Garabedian; Francisco Fernandez-Lima; Fenfei Leng
Journal:  PLoS One       Date:  2015-06-26       Impact factor: 3.240

7.  Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays.

Authors:  Linjia Su; Nadezda Bryan; Sabrina Battista; Juliano Freitas; Alyssa Garabedian; Federica D'Alessio; Miriam Romano; Fabiana Falanga; Alfredo Fusco; Lidia Kos; Jeremy Chambers; Francisco Fernandez-Lima; Prem P Chapagain; Stefan Vasile; Layton Smith; Fenfei Leng
Journal:  Sci Rep       Date:  2020-11-02       Impact factor: 4.379
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.