Literature DB >> 1726329

Assays for testing Pneumocystis carinii viability.

E S Kaneshiro1, Y P Wu, M T Cushion.   

Abstract

A series of classical vital stains and fluorescent indicator compounds were evaluated as viability assays of P. carinii. The combination of the acetoxymethyl ester of calcein with either ethidium homodimer or propidium iodide distinguished between live, dead and moribund organisms and provided high fluorescence intensity and low bleaching enabling photodocumentation at high magnifications.

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Year:  1991        PMID: 1726329

Source DB:  PubMed          Journal:  J Protozool        ISSN: 0022-3921


  7 in total

1.  Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol.

Authors:  R López-Amorós; J Comas; J Vives-Rego
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

2.  Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations.

Authors:  J A De Stefano; J M Foy; D W Sullivan; S M Dawes; M T Cushion; G F Babcock; R G Sleight; H van Halbeek; P D Walzer
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

3.  C27 to C32 sterols found in Pneumocystis, an opportunistic pathogen of immunocompromised mammals.

Authors:  E S Kaneshiro; M A Wyder
Journal:  Lipids       Date:  2000-03       Impact factor: 1.880

Review 4.  The lipids of Pneumocystis carinii.

Authors:  E S Kaneshiro
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

5.  Use of an ATP bioluminescent assay to evaluate viability of Pneumocystis carinii from rats.

Authors:  F Chen; M T Cushion
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

6.  Active immunity to Pneumocystis carinii reinfection in T-cell-depleted mice.

Authors:  A G Harmsen; W Chen; F Gigliotti
Journal:  Infect Immun       Date:  1995-07       Impact factor: 3.441

7.  SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs.

Authors:  Annie Standaert-Vitse; Cécile-Marie Aliouat-Denis; Anna Martinez; Sara Khalife; Muriel Pottier; Nausicaa Gantois; Eduardo Dei-Cas; El Moukhtar Aliouat
Journal:  PLoS One       Date:  2015-06-23       Impact factor: 3.240

  7 in total

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