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Literature DB >> 17261498

Establishment of an arsenic trioxide-resistant human leukemia cell line that shows multidrug resistance.

Tamami Seo¹, Yoshimasa Urasaki, Takanori Ueda.

Abstract

We have established an arsenic trioxide (As2O3)-resistant cell line (K562/AS-3) derived from the human leukemia cell line K562. K562/AS-3 was sequentially cultured with increasing concentrations of As2O3 up to 3.5 microM and then cloned by the limiting dilution method. K562/AS-3 was found to be about 7-fold more resistant to As2O3 than the parent cells (IC50=12.9 microM for K562/AS-3 and 1.8 microM for K562), and also showed cross resistance to VP-16 and vincristine. The multidrug resistance-associated protein (MRP1) gene was found to be overexpressed, but the MDR gene was not detected. MRP1 function was evaluated by measuring calcein acetoxymethyl ester (calcein-AM) efflux, and by verifying its inhibition by MK571, a potent MRP inhibitor. In addition, an increase of the total intracellular glutathione content was found in K562/AS-3. The resistance of K562/AS-3 to As2O3 was reversed by the addition of MK571, but not by verapamil. K562/AS-3 may be useful for studying the mechanism of the anticancer effect of As2O3 and how to overcome As2O3-resistance.

Entities: CellLine Chemical Disease Gene Species

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Year: 2007 PMID： 17261498 DOI： 10.1532/IJH97.05142

Source DB: PubMed Journal: Int J Hematol ISSN： 0925-5710 Impact factor: 2.490

31 in total

1. Involvement of CD95-independent caspase 8 activation in arsenic trioxide-induced apoptosis.

Authors: K Kitamura; Y Minami; K Yamamoto; Y Akao; H Kiyoi; H Saito; T Naoe
Journal: Leukemia Date: 2000-10 Impact factor: 11.528

2. Malignant cells can be sensitized to undergo growth inhibition and apoptosis by arsenic trioxide through modulation of the glutathione redox system.

Authors: J Dai; R S Weinberg; S Waxman; Y Jing
Journal: Blood Date: 1999-01-01 Impact factor: 22.113

3. Arsenic trioxide-induced apoptosis through oxidative stress in cells of colon cancer cell lines.

Authors: Yoshihito Nakagawa; Yukihiro Akao; Hiroshi Morikawa; Ichiro Hirata; Kenichi Katsu; Tomoki Naoe; Nobuko Ohishi; Kunio Yagi
Journal: Life Sci Date: 2002-03-29 Impact factor: 5.037

4. Elevation of glutathione levels and glutathione S-transferase activity in arsenic-resistant Chinese hamster ovary cells.

Authors: T C Lee; M L Wei; W J Chang; I C Ho; J F Lo; K Y Jan; H Huang
Journal: In Vitro Cell Dev Biol Date: 1989-05

5. Chronic arsenic-exposed human prostate epithelial cells exhibit stable arsenic tolerance: mechanistic implications of altered cellular glutathione and glutathione S-transferase.

Authors: Eduardo M Brambila; William E Achanzar; Wei Qu; Mukta M Webber; Michael P Waalkes
Journal: Toxicol Appl Pharmacol Date: 2002-09-01 Impact factor: 4.219

6. Studies on treatment of acute promyelocytic leukemia with arsenic trioxide: remission induction, follow-up, and molecular monitoring in 11 newly diagnosed and 47 relapsed acute promyelocytic leukemia patients.

Authors: C Niu; H Yan; T Yu; H P Sun; J X Liu; X S Li; W Wu; F Q Zhang; Y Chen; L Zhou; J M Li; X Y Zeng; R R Yang; M M Yuan; M Y Ren; F Y Gu; Q Cao; B W Gu; X Y Su; G Q Chen; S M Xiong; T D Zhang; S Waxman; Z Y Wang; Z Chen; J Hu; Z X Shen; S J Chen
Journal: Blood Date: 1999-11-15 Impact factor: 22.113

7. In vitro studies on cellular and molecular mechanisms of arsenic trioxide (As2O3) in the treatment of acute promyelocytic leukemia: As2O3 induces NB4 cell apoptosis with downregulation of Bcl-2 expression and modulation of PML-RAR alpha/PML proteins.

Authors: G Q Chen; J Zhu; X G Shi; J H Ni; H J Zhong; G Y Si; X L Jin; W Tang; X S Li; S M Xong; Z X Shen; G L Sun; J Ma; P Zhang; T D Zhang; C Gazin; T Naoe; S J Chen; Z Y Wang; Z Chen
Journal: Blood Date: 1996-08-01 Impact factor: 22.113

8. Glutathione depletion overcomes resistance to arsenic trioxide in arsenic-resistant cell lines.

Authors: K Davison; S Côté; S Mader; W H Miller
Journal: Leukemia Date: 2003-05 Impact factor: 11.528

9. Immunochemical detection of the multidrug resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies.

Authors: M J Flens; M A Izquierdo; G L Scheffer; J M Fritz; C J Meijer; R J Scheper; G J Zaman
Journal: Cancer Res Date: 1994-09-01 Impact factor: 12.701

10. Arsenic trioxide sensitivity is associated with low level of glutathione in cancer cells.

Authors: C H Yang; M L Kuo; J C Chen; Y C Chen
Journal: Br J Cancer Date: 1999-11 Impact factor: 7.640

5 in total

1. Efflux Transporters Regulate Arsenite-Induced Genotoxicity in Double Negative and Double Positive T Cells.

Authors: Huan Xu; Sebastian Medina; Fredine T Lauer; Christelle Douillet; Ke Jian Liu; Laurie G Hudson; Miroslav Stýblo; Lauren M Aleksunes; Scott W Burchiel
Journal: Toxicol Sci Date: 2017-07-01 Impact factor: 4.849

2. The NRF2-mediated oxidative stress response pathway is associated with tumor cell resistance to arsenic trioxide across the NCI-60 panel.

Authors: Qian Liu; Hao Zhang; Lisa Smeester; Fei Zou; Matt Kesic; Ilona Jaspers; Jingbo Pi; Rebecca C Fry
Journal: BMC Med Genomics Date: 2010-08-13 Impact factor: 3.063