OBJECTIVE: To determine the inhibitory effects of all-trans-retinoic acid (ATRA) on cell growth, cell cycle and vascular endothelial growth factor (VEGF) expression in the human gastric cancer cell line BGC-823 in vitro. METHODS: Human gastric cancer BGC-823 cells were treated with various concentrations of ATRA and the cell growth was then determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide viability assay. The cell cycle distribution was analyzed using a flow cytometer. The VEGF mRNA and protein expression were analyzed by semi-quantitative RT-PCR and Western blotting, respectively. RESULTS: ATRA at concentrations of 0.1-10 micromol/L inhibited the growth of BGC-823 cells grown in culture; a time- and dose-dependent inhibitory influence was found. ATRA arrested BGC-823 cells at the G0/G1 phase in a dose-dependent way. Both VEGF mRNA and protein were decreased by ATRA in a dose-dependent way. CONCLUSION: The anti-tumor effects of ATRA on human gastric cancer cells are associated with G0/G1 phase arrest and decreased VEGF expression.
OBJECTIVE: To determine the inhibitory effects of all-trans-retinoic acid (ATRA) on cell growth, cell cycle and vascular endothelial growth factor (VEGF) expression in the humangastric cancer cell line BGC-823 in vitro. METHODS:Humangastric cancer BGC-823 cells were treated with various concentrations of ATRA and the cell growth was then determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide viability assay. The cell cycle distribution was analyzed using a flow cytometer. The VEGF mRNA and protein expression were analyzed by semi-quantitative RT-PCR and Western blotting, respectively. RESULTS:ATRA at concentrations of 0.1-10 micromol/L inhibited the growth of BGC-823 cells grown in culture; a time- and dose-dependent inhibitory influence was found. ATRA arrested BGC-823 cells at the G0/G1 phase in a dose-dependent way. Both VEGF mRNA and protein were decreased by ATRA in a dose-dependent way. CONCLUSION: The anti-tumor effects of ATRA on humangastric cancer cells are associated with G0/G1 phase arrest and decreased VEGF expression.
Authors: P H Nguyen; J Giraud; C Staedel; L Chambonnier; P Dubus; E Chevret; H Bœuf; X Gauthereau; B Rousseau; M Fevre; I Soubeyran; G Belleannée; S Evrard; D Collet; F Mégraud; C Varon Journal: Oncogene Date: 2016-05-09 Impact factor: 9.867
Authors: Neil Sidell; Yue Feng; Lijuan Hao; Juanjuan Wu; Jie Yu; Maureen A Kane; Joseph L Napoli; Robert N Taylor Journal: Mol Endocrinol Date: 2009-11-12