Literature DB >> 17261046

Application of thiolated gold nanoparticles for the enhancement of glucose oxidase activity.

Pratibha Pandey1, Surinder P Singh, Sunil K Arya, Vinay Gupta, Monika Datta, Sukhvir Singh, Bansi D Malhotra.   

Abstract

Glucose oxidase (GOx) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles (5-8 nm) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lower value of the Michaelis-Menton constant obtained for the immobilized (3.74 mM) GOx compared with that for the free (5.85 mM) GOx suggests significant enhancement in the activity of GOx attached to thiolated gold nanoparticles. The covalently immobilized GOx thiolated nanoparticles exhibit a response time of 30 s, a shelf life of more than 6 months, and improved tolerance to both pH and temperature.

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Year:  2007        PMID： 17261046     DOI： 10.1021/la062901c

Source DB:  PubMed          Journal:  Langmuir        ISSN： 0743-7463            Impact factor:   3.882


  18 in total

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