| Literature DB >> 17259991 |
Nicola Heim1, Olga Garaschuk, Michael W Friedrich, Marco Mank, Ruxandra I Milos, Yury Kovalchuk, Arthur Konnerth, Oliver Griesbeck.
Abstract
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.Entities:
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Year: 2007 PMID： 17259991 DOI： 10.1038/nmeth1009
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547