Literature DB >> 17258338

A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms.

Rudolf Gmür1, Helga Lüthi-Schaller.   

Abstract

A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest.

Mesh：

Year:  2006        PMID： 17258338     DOI： 10.1016/j.mimet.2006.12.012

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

1.  Biogeography of a human oral microbiome at the micron scale.

Authors:  Jessica L Mark Welch; Blair J Rossetti; Christopher W Rieken; Floyd E Dewhirst; Gary G Borisy
Journal:  Proc Natl Acad Sci U S A       Date:  2016-01-25       Impact factor: 11.205

2.  Episymbiotic Saccharibacteria suppresses gingival inflammation and bone loss in mice through host bacterial modulation.

Authors:  Otari Chipashvili; Daniel R Utter; Joseph K Bedree; Yansong Ma; Fabian Schulte; Gabrielle Mascarin; Yasmin Alayyoubi; Deepak Chouhan; Markus Hardt; Felicitas Bidlack; Hatice Hasturk; Xuesong He; Jeffrey S McLean; Batbileg Bor
Journal:  Cell Host Microbe       Date:  2021-10-11       Impact factor: 21.023

3.  Oral biofilm architecture on natural teeth.

Authors:  Vincent Zijnge; M Barbara M van Leeuwen; John E Degener; Frank Abbas; Thomas Thurnheer; Rudolf Gmür; Hermie J M Harmsen
Journal:  PLoS One       Date:  2010-02-24       Impact factor: 3.240

4.  Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms.

Authors:  Beatrice Quevedo; Elin Giertsen; Vincent Zijnge; Helga Lüthi-Schaller; Bernhard Guggenheim; Thomas Thurnheer; Rudolf Gmür
Journal:  BMC Microbiol       Date:  2011-01-19       Impact factor: 3.605
  4 in total

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