OBJECTIVE: Allogeneic hematopoietic stem cell transplantation (allo-HCT) is frequently complicated by severe infections and graft-vs-host disease (GVHD). Saliva contains many components of adaptive and innate immune response crucial for local host defenses. Changes in salivary constituents could reflect systemic processes such as immune reconstitution and development of GVHD that occur posttransplant. This study was an initial evaluation of salivary protein changes that occur after allo-HCT. PATIENTS AND METHODS: Serially collected saliva samples from 41 patients undergoing allo-HCT were evaluated. Changes in salivary proteome were initially examined by SELDI-TOF mass spectrometry. Individual protein changes were identified by 2-dimensional differential in-gel electrophoresis (2D-DIGE) with subsequent MS/MS sequencing and ELISA. RESULTS: Significant increases and decreases in multiple salivary proteins that lasted at least 2 months posttransplant were detected by SELDI-TOF mass spectrometry. Lactoferrin and secretory leukocyte protease inhibitor demonstrated elevations 1 month post-HCT that persisted at least 6 months. Secretory IgA (sIgA) levels were decreased 1 month posttransplant, with recovery at approximately 6 months. Levels of salivary beta(2)-microglobulin were elevated at 6 months and correlated with sIgA levels. CONCLUSION: Allo-HCT is associated with long-term changes in several salivary proteins important for innate immune responses. These results support further studies on the association of salivary proteins with posttransplant complications including infections and GVHD.
OBJECTIVE: Allogeneic hematopoietic stem cell transplantation (allo-HCT) is frequently complicated by severe infections and graft-vs-host disease (GVHD). Saliva contains many components of adaptive and innate immune response crucial for local host defenses. Changes in salivary constituents could reflect systemic processes such as immune reconstitution and development of GVHD that occur posttransplant. This study was an initial evaluation of salivary protein changes that occur after allo-HCT. PATIENTS AND METHODS: Serially collected saliva samples from 41 patients undergoing allo-HCT were evaluated. Changes in salivary proteome were initially examined by SELDI-TOF mass spectrometry. Individual protein changes were identified by 2-dimensional differential in-gel electrophoresis (2D-DIGE) with subsequent MS/MS sequencing and ELISA. RESULTS: Significant increases and decreases in multiple salivary proteins that lasted at least 2 months posttransplant were detected by SELDI-TOF mass spectrometry. Lactoferrin and secretory leukocyte protease inhibitor demonstrated elevations 1 month post-HCT that persisted at least 6 months. Secretory IgA (sIgA) levels were decreased 1 month posttransplant, with recovery at approximately 6 months. Levels of salivary beta(2)-microglobulin were elevated at 6 months and correlated with sIgA levels. CONCLUSION: Allo-HCT is associated with long-term changes in several salivary proteins important for innate immune responses. These results support further studies on the association of salivary proteins with posttransplant complications including infections and GVHD.
Authors: G E Sale; H M Shulman; M M Schubert; K M Sullivan; K J Kopecky; R C Hackman; T H Morton; R Storb; E D Thomas Journal: Hum Pathol Date: 1981-11 Impact factor: 3.466
Authors: Thorsten Kaiser; Haytham Kamal; Andreas Rank; Hans-Jochem Kolb; Ernst Holler; Arnold Ganser; Bernd Hertenstein; Harald Mischak; Eva Maria Weissinger Journal: Blood Date: 2004-03-30 Impact factor: 22.113
Authors: Ernst Holler; Gerhard Rogler; Hans Herfarth; Julia Brenmoehl; Peter Johannes Wild; Joachim Hahn; Günther Eissner; Jürgen Schölmerich; Reinhard Andreesen Journal: Blood Date: 2004-04-15 Impact factor: 22.113
Authors: Sompop Bencharit; Sandra K Altarawneh; Sarah Schwartz Baxter; Jim Carlson; Gary F Ross; Michael B Border; C Russell Mack; Warren C Byrd; Christopher F Dibble; Silvana Barros; Zvi Loewy; Steven Offenbacher Journal: Mol Biosyst Date: 2012-10-30
Authors: Joel B Epstein; Judith E Raber-Durlacher; Michael Lill; Yuliya P L Linhares; Jerry Chang; Andrei Barasch; Romée I C Slief; Margot Geuke; Judith A E M Zecha; Dan M J Milstein; D Tzachanis Journal: Support Care Cancer Date: 2016-09-21 Impact factor: 3.603
Authors: Fatima Ardito; Donatella Perrone; Roberto Cocchi; Lucio Lo Russo; Alfredo DE Lillo; Giovanni Giannatempo; Lorenzo Lo Muzio Journal: Oncol Lett Date: 2016-02-09 Impact factor: 2.967