AIMS: Despite the interest of phyllosphere microbiology, no studies have addressed the bacteria present in bract phyllosphere, an ecosystem that has special characteristics in palm trees because the dry bracts remain on the plant until pruning and may contain polymer-degrading bacteria involved in plant degradation. Therefore, the aim of this work was to characterize xylanolytic bacteria isolated from palm bract phyllosphere. METHODS AND RESULTS: Twelve xylanolytic strains were isolated and characterized by phenotypic features and complete sequencing of 16S rRNA gene. The results showed that the isolates were phenotypically and genotypically diverse. Gram-positive isolates were classified into genus Paenibacillus some of them belonging to hitherto undescribed species of this genus. Gram-negative isolates were classified into genera Pseudomonas and Acinetobacter. CONCLUSIONS: The results of this work confirm the complexity of the bacterial populations present in phyllospheric ecosystems and suggest that bacteria involved in plant degradation are present at the early degradation steps of this process in dry palm tree bracts. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study on bract phyllospheric bacteria able to hydrolyse vegetal polymers and offers a new perspective in the search of unexplored sources of xylanase-producing strains.
AIMS: Despite the interest of phyllosphere microbiology, no studies have addressed the bacteria present in bract phyllosphere, an ecosystem that has special characteristics in palm trees because the dry bracts remain on the plant until pruning and may contain polymer-degrading bacteria involved in plant degradation. Therefore, the aim of this work was to characterize xylanolytic bacteria isolated from palm bract phyllosphere. METHODS AND RESULTS: Twelve xylanolytic strains were isolated and characterized by phenotypic features and complete sequencing of 16S rRNA gene. The results showed that the isolates were phenotypically and genotypically diverse. Gram-positive isolates were classified into genus Paenibacillus some of them belonging to hitherto undescribed species of this genus. Gram-negative isolates were classified into genera Pseudomonas and Acinetobacter. CONCLUSIONS: The results of this work confirm the complexity of the bacterial populations present in phyllospheric ecosystems and suggest that bacteria involved in plant degradation are present at the early degradation steps of this process in dry palm tree bracts. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study on bract phyllospheric bacteria able to hydrolyse vegetal polymers and offers a new perspective in the search of unexplored sources of xylanase-producing strains.
Authors: Marta Marcos-García; Paula García-Fraile; Alena Filipová; Esther Menéndez; Pedro F Mateos; Encarna Velázquez; Tomáš Cajthaml; Raúl Rivas Journal: Environ Sci Pollut Res Int Date: 2017-06-07 Impact factor: 4.223
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Authors: Márcia Bacelar Fonseca; Alvaro Peix; Sergio Miana de Faria; Pedro F Mateos; Lina P Rivera; Jean L Simões-Araujo; Marcel Giovanni Costa França; Rosy Mary dos Santos Isaias; Cristina Cruz; Encarna Velázquez; Maria Rita Scotti; Janet I Sprent; Euan K James Journal: PLoS One Date: 2012-11-19 Impact factor: 3.240
Authors: Zaki Saati-Santamaría; Rubén López-Mondéjar; Alejandro Jiménez-Gómez; Alexandra Díez-Méndez; Tomáš Větrovský; José M Igual; Encarna Velázquez; Miroslav Kolarik; Raúl Rivas; Paula García-Fraile Journal: Front Microbiol Date: 2018-05-08 Impact factor: 5.640