Michael S Stosich1, Jeremy J Mao. 1. New York, N.Y. From the College of Dental Medicine and Department of Biomedical Engineering, Columbia University.
Abstract
BACKGROUND: Despite certain levels of clinical efficacy, current autografts and synthetic materials for soft-tissue reconstruction and/or augmentation suffer from donor-site morbidity, rupture, dislocation, and volume reduction. Human adult stem cells can self-replicate and differentiate into adipogenic cells in response to appropriate signaling cues. This study investigated the shape and dimension maintenance of engineered adipose tissue from adult human mesenchymal stem cells. METHODS: Human mesenchymal stem cells were isolated from bone marrow of a healthy donor and differentiated into adipogenic cells. Adipocytes derived from these cells were encapsulated in a poly(ethylene glycol)-based hydrogel shaped into a generic cylinder (n = 6 implants), with hydrogel encapsulating human mesenchymal stem cells (n = 6) and cell-free hydrogel (n = 6) as controls. Porous collagen sponges were also used to seed human mesenchymal stem cell-derived adipocytes (n = 6), human mesenchymal stem cells (n = 4), or without cells (n = 4). All poly(ethylene glycol) and collagen constructs were implanted subcutaneously in athymic mice for 4 weeks. RESULTS: In vivo grafts demonstrated the formation of substantial adipose tissue encapsulating human mesenchymal stem cell-derived adipogenic cells in either poly(ethylene glycol)-based hydrogel or collagen sponge and a lack of adipose tissue formation in cell-free or human mesenchymal stem cell-derived grafts. Engineered adipose tissue in poly(ethylene glycol)-based hydrogel maintained approximately 100 percent of the original dimensions after 4-week in vivo implantation, significantly higher than the approximately 35 to 65 percent volume retention by collagen sponge. CONCLUSIONS: These findings demonstrate that the predefined shape and dimensions of adipose tissue engineered from human mesenchymal stem cells can be maintained after in vivo implantation. These data further indicate the potential for autologous applications in reconstructive and plastic surgery procedures.
BACKGROUND: Despite certain levels of clinical efficacy, current autografts and synthetic materials for soft-tissue reconstruction and/or augmentation suffer from donor-site morbidity, rupture, dislocation, and volume reduction. Human adult stem cells can self-replicate and differentiate into adipogenic cells in response to appropriate signaling cues. This study investigated the shape and dimension maintenance of engineered adipose tissue from adult human mesenchymal stem cells. METHODS:Human mesenchymal stem cells were isolated from bone marrow of a healthy donor and differentiated into adipogenic cells. Adipocytes derived from these cells were encapsulated in a poly(ethylene glycol)-based hydrogel shaped into a generic cylinder (n = 6 implants), with hydrogel encapsulating human mesenchymal stem cells (n = 6) and cell-free hydrogel (n = 6) as controls. Porous collagen sponges were also used to seed human mesenchymal stem cell-derived adipocytes (n = 6), human mesenchymal stem cells (n = 4), or without cells (n = 4). All poly(ethylene glycol) and collagen constructs were implanted subcutaneously in athymic mice for 4 weeks. RESULTS: In vivo grafts demonstrated the formation of substantial adipose tissue encapsulating human mesenchymal stem cell-derived adipogenic cells in either poly(ethylene glycol)-based hydrogel or collagen sponge and a lack of adipose tissue formation in cell-free or human mesenchymal stem cell-derived grafts. Engineered adipose tissue in poly(ethylene glycol)-based hydrogel maintained approximately 100 percent of the original dimensions after 4-week in vivo implantation, significantly higher than the approximately 35 to 65 percent volume retention by collagen sponge. CONCLUSIONS: These findings demonstrate that the predefined shape and dimensions of adipose tissue engineered from human mesenchymal stem cells can be maintained after in vivo implantation. These data further indicate the potential for autologous applications in reconstructive and plastic surgery procedures.
Authors: Adel Alhadlaq; Jennifer H Elisseeff; Liu Hong; Christopher G Williams; Arnold I Caplan; Blanka Sharma; Ross A Kopher; Sara Tomkoria; Donald P Lennon; Aurora Lopez; Jeremy J Mao Journal: Ann Biomed Eng Date: 2004-07 Impact factor: 3.934
Authors: Ricardo Sanz-Ruiz; María Eugenia Fernández Santos; Marta Domínguez Muñoa; Ingrid Ludwig Martín; Radoslaw Parma; Pedro L Sánchez Fernández; Francisco Fernández-Avilés Journal: J Cardiovasc Transl Res Date: 2008-01-29 Impact factor: 4.132
Authors: Michael S Stosich; Eduardo K Moioli; June K Wu; Chang Hun Lee; Christine Rohde; Azizeh Mitra Yoursef; Jeffrey Ascherman; Robert Diraddo; Nicholas W Marion; Jeremy J Mao Journal: Methods Date: 2008-10-24 Impact factor: 3.608
Authors: Jeremy J Mao; Michael S Stosich; Eduardo K Moioli; Chang Hun Lee; Susan Y Fu; Barbara Bastian; Sidney B Eisig; Candice Zemnick; Jeffrey Ascherman; June Wu; Christine Rohde; Jeffrey Ahn Journal: Tissue Eng Part B Rev Date: 2010-04 Impact factor: 6.389