Christina L Aquilante1, Amber L Beitelshees, Issam Zineh. 1. Department of Pharmaceutical Sciences, University of Colorado at Denver and Health Sciences Center, School of Pharmacy, Denver, Colorado, USA. Christina.aquilante@UCHSC.edu
Abstract
BACKGROUND: Matrix metalloproteinase-8 (MMP-8) has been implicated in the pathogenesis of cardiovascular disease. We evaluated the relative contribution of genetic and non-genetic variables to serum MMP-8 concentrations in nondiabetic subjects without known cardiovascular disease. METHODS: A blood sample was obtained from 100 subjects > or =18 y. Total serum MMP-8 concentrations were assayed by ELISA. MMP8 genotypes (-799 C/T and -381 A/G) were determined by PCR-pyrosequencing. RESULTS: MMP-8 concentrations were significantly higher in subjects with the metabolic syndrome (n=40) compared to those without the metabolic syndrome (11.71 vs 6.81 ng/ml, p<0.001) and in current smokers compared to non-smokers (13.51 vs 7.53 ng/ml, p=0.001). MMP-8 concentrations were significantly correlated with body mass index, triglyceride/HDL ratio, triglycerides, fasting plasma glucose, and age. MMP-8 concentrations were not significantly different between MMP8 genotype groups. In regression analysis, variables that were significantly associated with serum MMP-8 concentrations were presence of the metabolic syndrome and smoking (p<0.001 and p=0.009, respectively), accounting for 21.4% of the variability in serum MMP-8 concentrations. CONCLUSIONS: Our data demonstrate that the metabolic syndrome and smoking are independently associated with elevated serum MMP-8 concentrations. The relationship between MMP-8 concentrations and cardiovascular risk merits further investigation.
BACKGROUND:Matrix metalloproteinase-8 (MMP-8) has been implicated in the pathogenesis of cardiovascular disease. We evaluated the relative contribution of genetic and non-genetic variables to serum MMP-8 concentrations in nondiabetic subjects without known cardiovascular disease. METHODS: A blood sample was obtained from 100 subjects > or =18 y. Total serum MMP-8 concentrations were assayed by ELISA. MMP8 genotypes (-799 C/T and -381 A/G) were determined by PCR-pyrosequencing. RESULTS:MMP-8 concentrations were significantly higher in subjects with the metabolic syndrome (n=40) compared to those without the metabolic syndrome (11.71 vs 6.81 ng/ml, p<0.001) and in current smokers compared to non-smokers (13.51 vs 7.53 ng/ml, p=0.001). MMP-8 concentrations were significantly correlated with body mass index, triglyceride/HDL ratio, triglycerides, fasting plasma glucose, and age. MMP-8 concentrations were not significantly different between MMP8 genotype groups. In regression analysis, variables that were significantly associated with serum MMP-8 concentrations were presence of the metabolic syndrome and smoking (p<0.001 and p=0.009, respectively), accounting for 21.4% of the variability in serum MMP-8 concentrations. CONCLUSIONS: Our data demonstrate that the metabolic syndrome and smoking are independently associated with elevated serum MMP-8 concentrations. The relationship between MMP-8 concentrations and cardiovascular risk merits further investigation.
Authors: Derek L Mattey; Jonathan C Packham; Nicola B Nixon; Lucy Coates; Paul Creamer; Sarah Hailwood; Gordon J Taylor; Ashok K Bhalla Journal: Arthritis Res Ther Date: 2012-05-28 Impact factor: 5.156
Authors: Vanessa J Craig; Francesca Polverino; Maria E Laucho-Contreras; Yuanyuan Shi; Yushi Liu; Juan C Osorio; Yohannes Tesfaigzi; Victor Pinto-Plata; Bernadette R Gochuico; Ivan O Rosas; Caroline A Owen Journal: PLoS One Date: 2014-05-14 Impact factor: 3.240