Literature DB >> 172516

A simple method for freeze-fracture of monolayer cultures.

T R Collins, J C Bartholomew, M Calvin.   

Abstract

A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate.

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Year:  1975        PMID： 172516      PMCID： PMC2111649          DOI： 10.1083/jcb.67.3.904

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  11 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  Steps in the neoplastic transformation of hamster embryo cells by polyoma virus.

Authors:  M VOGT; R DULBECCO
Journal:  Proc Natl Acad Sci U S A       Date:  1963-02-15       Impact factor: 11.205

3.  Nutrition of animal cells in tissue culture; initial studies on a synthetic medium.

Authors:  J F MORGAN; H J MORTON; R C PARKER
Journal:  Proc Soc Exp Biol Med       Date:  1950-01

4.  Some artifacts of the freeze-etching technique.

Authors:  W F Dunlop; A W Robards
Journal:  J Ultrastruct Res       Date:  1972-08

5.  Glandular epithelial cells from mice: a method for selective cultivation.

Authors:  R B Owens
Journal:  J Natl Cancer Inst       Date:  1974-04       Impact factor: 13.506

6.  Recent progress in the freeze-etching technique.

Authors:  H Moor
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1971-05-27       Impact factor: 6.237

7.  Adaptation of the fluorescent Feulgen reaction to cells in suspension for flow microfluorometry.

Authors:  T T Trujillo; M A Van Dilla
Journal:  Acta Cytol       Date:  1972 Jan-Feb       Impact factor: 2.319

8.  Temperature and contamination dependent freeze-etch images of frozen water and glycerol solutions.

Authors:  L A Staehelin; W S Bertaud
Journal:  J Ultrastruct Res       Date:  1971-10

9.  Preferential inhibition of the growth of virus-transformed cells in culture by rifazone-82, a new rifamycin derivative.

Authors:  M J Bissell; C Hatie; A N Tischler; M Calvin
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

10.  Methods for the freeze-fracturing of nerve tissue cultures and cell monolayers.

Authors:  K H Pfenninger; E R Rinderer
Journal:  J Cell Biol       Date:  1975-04       Impact factor: 10.539
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  3 in total

1.  Freeze-fracture of monolayer cultures.

Authors:  B Pauli; R S Weinstein; L W Soble; J Alroy
Journal:  J Cell Biol       Date:  1977-03       Impact factor: 10.539

2.  A simple, selective method for freeze-fracturing spherical cells.

Authors:  H B Peng; L F Jaffe
Journal:  J Cell Biol       Date:  1976-11       Impact factor: 10.539

3.  Specializations in filopodial membranes at points of attachment to the substrate.

Authors:  J M Robinson; M J Karnovsky
Journal:  J Cell Biol       Date:  1980-12       Impact factor: 10.539
  3 in total

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