BACKGROUND/ PURPOSE: Porcine ear skin is used in studies of percutaneous penetration as a substitute for human skin. The structure of this tissue, including hair follicles, was studied qualitatively and quantitatively in comparison with human skin. METHODS: Sections of shock-frozen biopsies, biopsies embedded in paraffin and cyanoacrylate skin surface biopsies were investigated using microscopy. The thickness of the different skin layers and the follicular characteristics were determined. RESULTS: The thickness of the stratum corneum was 17-28 microm, whereas the viable epidermis was 60-85 microm thick. On 1 cm(2), 11-25 hairs were detected, showing a diameter of 58-97 microm and a maximal extension depth of 0.96-1.38 mm into the skin. The orifices of the porcine infundibula showed a diameter of approximately 200 microm. CONCLUSIONS: The results obtained are similar to those of human skin, indicating the suitability of this porcine tissue as a model for human skin.
BACKGROUND/ PURPOSE: Porcine ear skin is used in studies of percutaneous penetration as a substitute for human skin. The structure of this tissue, including hair follicles, was studied qualitatively and quantitatively in comparison with human skin. METHODS: Sections of shock-frozen biopsies, biopsies embedded in paraffin and cyanoacrylate skin surface biopsies were investigated using microscopy. The thickness of the different skin layers and the follicular characteristics were determined. RESULTS: The thickness of the stratum corneum was 17-28 microm, whereas the viable epidermis was 60-85 microm thick. On 1 cm(2), 11-25 hairs were detected, showing a diameter of 58-97 microm and a maximal extension depth of 0.96-1.38 mm into the skin. The orifices of the porcine infundibula showed a diameter of approximately 200 microm. CONCLUSIONS: The results obtained are similar to those of human skin, indicating the suitability of this porcine tissue as a model for human skin.
Authors: Abdullah Onul; Michael D Colvard; William A Paradise; Kim M Elseth; Benjamin J Vesper; Eftychia Gouvas; Zane Deliu; Kelly D Garcia; William J Pestle; James A Radosevich Journal: J Histochem Cytochem Date: 2012-06-21 Impact factor: 2.479
Authors: Mikolaj Milewski; Kalpana S Paudel; Nicole K Brogden; Priyanka Ghosh; Stan L Banks; Dana C Hammell; Audra L Stinchcomb Journal: Mol Pharm Date: 2013-09-23 Impact factor: 4.939