OBJECTIVE: The leaf extract of Cestrum parqui was examined for its effects on sperm motility in vitro. METHODS: Washed sperm were prepared by discontinuous buoyant density gradient centrifugation and incubated with varying concentrations of extract from C. parqui (40-250 microg/mL) at 37 degrees C and 5% CO(2). Sperm motility and viability were assessed at different intervals ranging from 5 to 240 min. Morphological changes in human ejaculated spermatozoa after exposure to the extract were evaluated under transmission electron microscope. RESULTS: A dose- and time-dependent effect of this extract on sperm motility and viability was observed. The maximal spermicidal effect was observed with a 250-microg/mL dose of the extract. Transmission electron microscope revealed a significant damage to sperm membrane in both head and acrosomal membranes, notable swelling and disruption. CONCLUSION: The present study indicates that this natural extract has potential spermicidal effect in vitro.
OBJECTIVE: The leaf extract of Cestrum parqui was examined for its effects on sperm motility in vitro. METHODS: Washed sperm were prepared by discontinuous buoyant density gradient centrifugation and incubated with varying concentrations of extract from C. parqui (40-250 microg/mL) at 37 degrees C and 5% CO(2). Sperm motility and viability were assessed at different intervals ranging from 5 to 240 min. Morphological changes in human ejaculated spermatozoa after exposure to the extract were evaluated under transmission electron microscope. RESULTS: A dose- and time-dependent effect of this extract on sperm motility and viability was observed. The maximal spermicidal effect was observed with a 250-microg/mL dose of the extract. Transmission electron microscope revealed a significant damage to sperm membrane in both head and acrosomal membranes, notable swelling and disruption. CONCLUSION: The present study indicates that this natural extract has potential spermicidal effect in vitro.
Authors: Edilson Damke; Joyce K Tsuzuki; Francieli Chassot; Diógenes A G Cortez; Izabel C P Ferreira; Cristiane S S Mesquita; Vânia R S da-Silva; Terezinha I E Svidzinski; Márcia E L Consolaro Journal: BMC Complement Altern Med Date: 2013-07-28 Impact factor: 3.659