Matthias Zehnder1, Gerhard Baumgartner, Klaus Marquardt, Frank Paqué. 1. Department of Preventive Dentistry, Periodontology and Cardiology, University of Zürich, Zürich, Switzerland. matthias.zehnder@zzmk.unizh.ch <matthias.zehnder@zzmk.unizh.ch>
Abstract
OBJECTIVE: The capacities of bioactive glass S53P4 and calcium hydroxide suspensions to prevent microbial leakage through instrumented root canals were compared. STUDY DESIGN: Root canals of caries-free extracted human mandibular premolars were instrumented and randomly filled with calcium hydroxide or S53P4 (n = 14 each). Crowns were exposed to Enterococcus faecalis ATCC 29212 suspensions in a test setup with 2 broth chambers, and leakage was checked daily. Event times were compared using log rank test. After 50 days, teeth were fractured and inspected in a scanning electron microscope (SEM). RESULTS: Calcium hydroxide prevented leakage significantly better than S53P4 (P < .05). Estimated mean days to leakage were 26 for calcium hydroxide and 4 days for S53P4. The SEM observations revealed substantial calcification of the glass material in the root canal but also gaps containing clusters of coccoidal bacteria. CONCLUSION: The bioactive glass material under investigation could not prevent contamination of instrumented root canals.
OBJECTIVE: The capacities of bioactive glass S53P4 and calcium hydroxide suspensions to prevent microbial leakage through instrumented root canals were compared. STUDY DESIGN: Root canals of caries-free extracted human mandibular premolars were instrumented and randomly filled with calcium hydroxide or S53P4 (n = 14 each). Crowns were exposed to Enterococcus faecalis ATCC 29212 suspensions in a test setup with 2 broth chambers, and leakage was checked daily. Event times were compared using log rank test. After 50 days, teeth were fractured and inspected in a scanning electron microscope (SEM). RESULTS:Calcium hydroxide prevented leakage significantly better than S53P4 (P < .05). Estimated mean days to leakage were 26 for calcium hydroxide and 4 days for S53P4. The SEM observations revealed substantial calcification of the glass material in the root canal but also gaps containing clusters of coccoidal bacteria. CONCLUSION: The bioactive glass material under investigation could not prevent contamination of instrumented root canals.
Authors: Ana Maria Hecke Alves; Maria Helena Pozzobon; Eduardo Antunes Bortoluzzi; Cleonice da Silveira Teixeira; Beatriz Dulcinéia Mendes Souza; Lucas da Fonseca Roberti Garcia; Wilson Tadeu Felippe Journal: Clin Oral Investig Date: 2017-09-15 Impact factor: 3.573