OBJECTIVE: Placental insufficiency is a primary cause of intrauterine growth restriction (IUGR). In our study, microarray technology was used to identify genes, which may impair placentation resulting in IUGR. STUDY DESIGN: The RNA was isolated from both IUGR term placentas and normal term placentas. Microarray experiments were used to identify differentially expressed genes between the 2 cohorts. Real-time quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry were used in follow-up experiments. RESULTS: Microarray experiments identified increased expression of certain genes including leptin, soluble vascular endothelial growth factor receptor, human chorionic gonadotropin, follistatin-like 3, and hypoxia-inducible factor 2alpha in the IUGR. Real-time quantitative polymerase chain reaction confirmed these results. CONCLUSION: The upregulation of soluble vascular endothelial growth factor receptor and hypoxia-inducible factor 2alpha at this period in pregnancy indicate that placental angiogenesis is altered in IUGR and that hypoxia is a major contributor to maldevelopment of the placental vasculature.
OBJECTIVE: Placental insufficiency is a primary cause of intrauterine growth restriction (IUGR). In our study, microarray technology was used to identify genes, which may impair placentation resulting in IUGR. STUDY DESIGN: The RNA was isolated from both IUGR term placentas and normal term placentas. Microarray experiments were used to identify differentially expressed genes between the 2 cohorts. Real-time quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry were used in follow-up experiments. RESULTS: Microarray experiments identified increased expression of certain genes including leptin, soluble vascular endothelial growth factor receptor, human chorionic gonadotropin, follistatin-like 3, and hypoxia-inducible factor 2alpha in the IUGR. Real-time quantitative polymerase chain reaction confirmed these results. CONCLUSION: The upregulation of soluble vascular endothelial growth factor receptor and hypoxia-inducible factor 2alpha at this period in pregnancy indicate that placental angiogenesis is altered in IUGR and that hypoxia is a major contributor to maldevelopment of the placental vasculature.
Authors: Julio Mateus; Roger B Newman; Cuilin Zhang; Sarah J Pugh; Jagteshwar Grewal; Sungduk Kim; William A Grobman; John Owen; Anthony C Sciscione; Ronald J Wapner; Daniel Skupski; Edward Chien; Deborah A Wing; Angela C Ranzini; Michael P Nageotte; Nicole Gerlanc; Paul S Albert; Katherine L Grantz Journal: Am J Obstet Gynecol Date: 2019-06-19 Impact factor: 8.661
Authors: S Brouillet; P Murthi; P Hoffmann; A Salomon; F Sergent; P De Mazancourt; M Dakouane-Giudicelli; M N Dieudonné; P Rozenberg; D Vaiman; S Barbaux; M Benharouga; J-J Feige; N Alfaidy Journal: Cell Mol Life Sci Date: 2012-09-02 Impact factor: 9.261
Authors: Elizabeth M Kennedy; Karen Hermetz; Amber Burt; Todd M Everson; Maya Deyssenroth; Ke Hao; Jia Chen; Margaret R Karagas; Dong Pei; Devin C Koestler; Carmen J Marsit Journal: Epigenetics Date: 2020-10-04 Impact factor: 4.528