OBJECTIVE: This study was designed to determine the optimal time point for bone marrow mesenchymal stem cell (MSC) transplantation after myocardial infarction (MI). METHODS: MSCs from donor rats were labeled with DAPI before transplantation. The animals underwent MI by ligation of left anterior descending coronary artery, and received intramyocardial injection of MSCs at 1h, 1 week and 2 weeks after MI, respectively. Sham-operated and MI control groups received equal volume phosphate buffered saline. Cardiac function, histological analysis and immunoblot for troponin T were performed 4 weeks after cell transplantation. RESULTS: MSC transplantation attenuated left ventricular chamber dilation, reduced infarct size, and improved cardiac function in rats after MI. The greatest benefit was achieved in rats that received cells 1 week after MI, engrafted MSC survival, angiogenesis and functional cardiomyocytes in the injured hearts were more abundant in these rats than that in other transplantation groups. CONCLUSIONS: The optimal functional benefit of MSC transplantation was observed in 1-week transplantation group. At this time point scar formation has not occurred and the inflammation is reduced, which should facilitate integration of transplanted cells and functional recovery.
OBJECTIVE: This study was designed to determine the optimal time point for bone marrow mesenchymal stem cell (MSC) transplantation after myocardial infarction (MI). METHODS: MSCs from donorrats were labeled with DAPI before transplantation. The animals underwent MI by ligation of left anterior descending coronary artery, and received intramyocardial injection of MSCs at 1h, 1 week and 2 weeks after MI, respectively. Sham-operated and MI control groups received equal volume phosphate buffered saline. Cardiac function, histological analysis and immunoblot for troponin T were performed 4 weeks after cell transplantation. RESULTS: MSC transplantation attenuated left ventricular chamber dilation, reduced infarct size, and improved cardiac function in rats after MI. The greatest benefit was achieved in rats that received cells 1 week after MI, engrafted MSC survival, angiogenesis and functional cardiomyocytes in the injured hearts were more abundant in these rats than that in other transplantation groups. CONCLUSIONS: The optimal functional benefit of MSC transplantation was observed in 1-week transplantation group. At this time point scar formation has not occurred and the inflammation is reduced, which should facilitate integration of transplanted cells and functional recovery.
Authors: Martin Rubach; Roland Adelmann; Moritz Haustein; Florian Drey; Kurt Pfannkuche; Bing Xiao; Annette Koester; Floris E A Udink ten Cate; Yeong-Hoon Choi; Klaus Neef; Azra Fatima; Tobias Hannes; Frank Pillekamp; Juergen Hescheler; Tomo Šarić; Konrad Brockmeier; Markus Khalil Journal: Stem Cells Dev Date: 2014-01-15 Impact factor: 3.272
Authors: P Locatelli; F D Olea; A Hnatiuk; A De Lorenzi; M Cerdá; C S Giménez; D Sepúlveda; R Laguens; A Crottogini Journal: Gene Ther Date: 2015-03-19 Impact factor: 5.250
Authors: Rutger-Jan Swijnenburg; Johannes A Govaert; Koen E A van der Bogt; Jeremy I Pearl; Mei Huang; William Stein; Grant Hoyt; Hannes Vogel; Christopher H Contag; Robert C Robbins; Joseph C Wu Journal: Circ Cardiovasc Imaging Date: 2009-11-17 Impact factor: 7.792