BACKGROUND: The venom of the spider Loxosceles can cause both local and systemic effects including disseminated intravascular coagulation. AIM: The aim of this study was to investigate the effects of the venom of Loxosceles intermedia (L. intermedia) and the purified Sphingomyelinase D (SMaseD) toxin upon the Protein C (PC) natural anticoagulant pathway. RESULTS: Both the venom and e purified SMaseD reduced the cell surface expression of thrombomodulin (TM) and Endothelial PC Receptor on endothelial cells in culture. The reduction of cell surface expression was caused by cleavage from the cell surface mediated by activation of an endogenous metalloproteinase. Reduction of TM and Endothelial PC Receptor on the surface of these cells resulted in an impaired ability of the cells to assist in the thrombin-induced activation of PC. CONCLUSION: This novel observation gives further insight into the mechanisms of the pathology induced by venom from Loxosceles spiders and may aid the development of a suitable therapy.
BACKGROUND: The venom of the spider Loxosceles can cause both local and systemic effects including disseminated intravascular coagulation. AIM: The aim of this study was to investigate the effects of the venom of Loxosceles intermedia (L. intermedia) and the purified Sphingomyelinase D (SMaseD) toxin upon the Protein C (PC) natural anticoagulant pathway. RESULTS: Both the venom and e purified SMaseD reduced the cell surface expression of thrombomodulin (TM) and Endothelial PC Receptor on endothelial cells in culture. The reduction of cell surface expression was caused by cleavage from the cell surface mediated by activation of an endogenous metalloproteinase. Reduction of TM and Endothelial PC Receptor on the surface of these cells resulted in an impaired ability of the cells to assist in the thrombin-induced activation of PC. CONCLUSION: This novel observation gives further insight into the mechanisms of the pathology induced by venom from Loxosceles spiders and may aid the development of a suitable therapy.
Authors: Wanessa Araújo Carvalho; Sandra Regina Maruyama; Alessandra Mara Franzin; Antônio Roberto Rodrigues Abatepaulo; Jennifer M Anderson; Beatriz Rossetti Ferreira; José Marcos Chaves Ribeiro; Daniela Dantas Moré; Antonio Augusto Mendes Maia; Jesus G Valenzuela; Gustavo Rocha Garcia; Isabel K Ferreira de Miranda Santos Journal: Exp Parasitol Date: 2010-01-04 Impact factor: 2.011
Authors: Rui V Lucato; Regina C R M Abdulkader; Katia C Barbaro; Glória E Mendes; Isac Castro; Maria A S F Baptista; Patrícia M Cury; Denise M C Malheiros; Nestor Schor; Luis Yu; Emmanuel A Burdmann Journal: PLoS Negl Trop Dis Date: 2011-05-31