Literature DB >> 1722666

Immunochemical analysis of high molecular-weight human salivary mucins (MG1) using monoclonal antibodies.

E C Veerman1, M Valentijn-Benz, P A van den Keybus, W M Rathman, J K Sheehan, A V Nieuw Amerongen.   

Abstract

Using four Mabs with different specificities for salivary mucins, an ELISA has been developed in which human whole saliva, glandular salivas, salivary protein fractions and purified, high molecular-weight, mucin fractions (MG1) isolated from human submandibular and sublingual glandular tissues have been immunochemically analysed. All four Mabs reacted with MG1s. Three of them reacted with the purified, low molecular-weight salivary mucins (MG2). None was reactive with parotid saliva. MG1 preparations isolated from submandibular and sublingual glandular tissues of one and the same individual displayed different patterns of reactivity with these Mabs, indicating that they differ immunochemically. Analysis of the MG1s in salivas derived from individual salivary glands showed differences in immunochemical composition. These results indicate that the MG1 fraction in human whole saliva consists of several immunochemically different species.

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Year:  1991        PMID： 1722666     DOI： 10.1016/0003-9969(91)90125-e

Source DB:  PubMed          Journal:  Arch Oral Biol        ISSN： 0003-9969            Impact factor:   2.633


  7 in total

1.  Binding of salivary agglutinin to IgA.

Authors:  Antoon J M Ligtenberg; Floris J Bikker; Jolanda M A De Blieck-Hogervorst; Enno C I Veerman; Arie V Nieuw Amerongen
Journal:  Biochem J       Date:  2004-10-01       Impact factor: 3.857

2.  Isolation of different high-Mr mucin species from human whole saliva.

Authors:  E C Veerman; P A van den Keybus; M Valentijn-Benz; A V Nieuw Amerongen
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

3.  Interaction of the salivary low-molecular-weight mucin (MG2) with Actinobacillus actinomycetemcomitans.

Authors:  J Groenink; A J Ligtenberg; E C Veerman; J G Bolscher; A V Nieuw Amerongen
Journal:  Antonie Van Leeuwenhoek       Date:  1996-07       Impact factor: 2.271

4.  Neutrophil-activating protein mediates adhesion of Helicobacter pylori to sulfated carbohydrates on high-molecular-weight salivary mucin.

Authors:  F Namavar; M Sparrius; E C Veerman; B J Appelmelk; C M Vandenbroucke-Grauls
Journal:  Infect Immun       Date:  1998-02       Impact factor: 3.441

5.  Influence of saliva on aggregation and adherence of Streptococcus gordonii HG 222.

Authors:  A J Ligtenberg; E Walgreen-Weterings; E C Veerman; J J de Soet; J de Graaff; A V Amerongen
Journal:  Infect Immun       Date:  1992-09       Impact factor: 3.441

6.  Distinct populations of high-M(r) mucins secreted by different human salivary glands discriminated by density-gradient electrophoresis.

Authors:  J Bolscher; E Veerman; A Van Nieuw Amerongen; A Tulp; D Verwoerd
Journal:  Biochem J       Date:  1995-08-01       Impact factor: 3.857

Review 7.  Salivary defense proteins: their network and role in innate and acquired oral immunity.

Authors:  Tibor Károly Fábián; Péter Hermann; Anita Beck; Pál Fejérdy; Gábor Fábián
Journal:  Int J Mol Sci       Date:  2012-04-02       Impact factor: 6.208
  7 in total

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