Literature DB >> 17223121

Quantitative analysis of bacterial medium-chain-length poly([R]-3-hydroxyalkanoates) by gas chromatography.

Patrick Furrer1, Roland Hany, Daniel Rentsch, Andreas Grubelnik, Katinka Ruth, Sven Panke, Manfred Zinn.   

Abstract

Standard chromatographic methods for the quantification of bacterial poly(3-hydroxyalkanoate) (PHA) proved to be inappropriate for the analysis of medium-chain-length PHA (mcl-PHA). Transesterification catalyzed by protic acids is not quantitative for mcl-PHA under common conditions due to slow reaction kinetics and formation of side-products in case of functionalized side-chains. To circumvent these limitations, an improved method for the quantification of mcl-PHA by GC-FID was developed. Boron trifluoride in methanol was successfully applied to quantitatively methanolyse different mcl-PHA (recovery >94%). This novel method is well-suited for the analysis of purified mcl-PHA as well as for mcl-PHA in biomass.

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Year:  2007        PMID： 17223121     DOI： 10.1016/j.chroma.2007.01.002

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  10 in total

1.  The influence of nitrogen limitation on mcl-PHA synthesis by two newly isolated strains of Pseudomonas sp.

Authors:  Slawomir Ciesielski; Justyna Mozejko; Grzegorz Przybyłek
Journal:  J Ind Microbiol Biotechnol       Date:  2010-03-04       Impact factor: 3.346

2.  The optimal combination of Nile red identification, colony polymerase chain reaction, and gas chromatography detection methods in screening for polyhydroxyalkanoicate-producing bacteria.

Authors:  Hung-Che Chou; Chia-Hsin Chen; Hsiao-Kai Chu; Chun-Mei Huang; Hui-Jun Wang; Wei-Lin Tu; Gia-Luen Guo
Journal:  Arch Microbiol       Date:  2022-05-10       Impact factor: 2.552

3.  A reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from gluconate.

Authors:  Stéphanie Follonier; Sven Panke; Manfred Zinn
Journal:  Microb Cell Fact       Date:  2011-04-22       Impact factor: 5.328

4.  Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB.

Authors:  Hannes Löwe; Karina Hobmeier; Manuel Moos; Andreas Kremling; Katharina Pflüger-Grau
Journal:  Biotechnol Biofuels       Date:  2017-07-19       Impact factor: 6.040

5.  The Bistable Behaviour of Pseudomonas putida KT2440 during PHA Depolymerization under Carbon Limitation.

Authors:  Stephanie Karmann; Sven Panke; Manfred Zinn
Journal:  Bioengineering (Basel)       Date:  2017-06-19

6.  Tailor-made PAT platform for safe syngas fermentations in batch, fed-batch and chemostat mode with Rhodospirillum rubrum.

Authors:  Stephanie Karmann; Stéphanie Follonier; Daniel Egger; Dirk Hebel; Sven Panke; Manfred Zinn
Journal:  Microb Biotechnol       Date:  2017-06-06       Impact factor: 5.813

Review 7.  Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization.

Authors:  Dana-Maria Miu; Mihaela Carmen Eremia; Misu Moscovici
Journal:  Materials (Basel)       Date:  2022-02-14       Impact factor: 3.623

8.  Production of a novel medium chain length poly(3-hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold.

Authors:  Pooja Basnett; Barbara Lukasiewicz; Elena Marcello; Harpreet K Gura; Jonathan C Knowles; Ipsita Roy
Journal:  Microb Biotechnol       Date:  2017-09-14       Impact factor: 5.813

9.  Exploring nutrient limitation for polyhydroxyalkanoates synthesis by newly isolated strains of Aeromonas sp. using biodiesel-derived glycerol as a substrate.

Authors:  Justyna Możejko-Ciesielska; Tomasz Pokoj
Journal:  PeerJ       Date:  2018-10-23       Impact factor: 2.984

10.  Influence of Unusual Co-substrates on the Biosynthesis of Medium-Chain-Length Polyhydroxyalkanoates Produced in Multistage Chemostat.

Authors:  Nils Hanik; Camila Utsunomia; Shuzo Arai; Ken'ichiro Matsumoto; Manfred Zinn
Journal:  Front Bioeng Biotechnol       Date:  2019-11-05
  10 in total

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