OBJECTIVES: Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. DESIGN AND METHODS: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. RESULTS AND CONCLUSIONS: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
OBJECTIVES:Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. DESIGN AND METHODS: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. RESULTS AND CONCLUSIONS: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
Authors: Flavia V Castelino; Gretchen Bain; Veronica A Pace; Katharine E Black; Leaya George; Clemens K Probst; Lance Goulet; Robert Lafyatis; Andrew M Tager Journal: Arthritis Rheumatol Date: 2016-12 Impact factor: 10.995
Authors: Luiz F Lisboa; Sonal Asthana; Andreas Kremer; Mark Swain; Sean M Bagshaw; Noel Gibney; Constantine J Karvellas Journal: Can J Gastroenterol Date: 2012-11 Impact factor: 3.522
Authors: Katharine E Black; Evgeny Berdyshev; Gretchen Bain; Flavia V Castelino; Barry S Shea; Clemens K Probst; Benjamin A Fontaine; Irina Bronova; Lance Goulet; David Lagares; Neil Ahluwalia; Rachel S Knipe; Viswanathan Natarajan; Andrew M Tager Journal: FASEB J Date: 2016-03-22 Impact factor: 5.191
Authors: Valerie L Reeves; Joy S Trybula; Rachel C Wills; Bret H Goodpaster; John J Dubé; Petra C Kienesberger; Erin E Kershaw Journal: Obesity (Silver Spring) Date: 2015-11-02 Impact factor: 5.002
Authors: Anna Michalczyk; Marta Budkowska; Barbara Dołęgowska; Dariusz Chlubek; Krzysztof Safranow Journal: Lipids Health Dis Date: 2017-07-21 Impact factor: 3.876