INTRODUCTION: The aim of the present study was to investigate the gas-dependent effects of pneumoperitoneum on wound healing distant from the abdomen. MATERIALS AND METHODS: Dorsal skin incisions were performed in 54 male Sprague-Dawley rats. A CO(2) or a helium pneumoperitoneum of 3 mmHg was maintained before, with an overall duration of 30 min (each n = 18). Rats in the control group received laparotomy only (n = 18). Animals were killed after 5 and 10 days. The infiltration of macrophages (CD 68), expression of matrix metalloproteinase 13 (MMP-13) and collagen I/III ratios were analysed in the dorsal skin wounds. RESULTS: Both after 5 and 10 days, infiltration of macrophages and expression of MMP-13 were greatest after helium pneumoperitoneum. After 5 days, collagen I/III ratio was significantly increased in the helium group. After 10 days, collagen I/III ratio was lowest in the CO(2) group. CONCLUSION: Our results suggest beneficial effects on systemic wound healing for helium pneumoperitoneum as compared to CO(2).
INTRODUCTION: The aim of the present study was to investigate the gas-dependent effects of pneumoperitoneum on wound healing distant from the abdomen. MATERIALS AND METHODS: Dorsal skin incisions were performed in 54 male Sprague-Dawley rats. A CO(2) or a helium pneumoperitoneum of 3 mmHg was maintained before, with an overall duration of 30 min (each n = 18). Rats in the control group received laparotomy only (n = 18). Animals were killed after 5 and 10 days. The infiltration of macrophages (CD 68), expression of matrix metalloproteinase 13 (MMP-13) and collagen I/III ratios were analysed in the dorsal skin wounds. RESULTS: Both after 5 and 10 days, infiltration of macrophages and expression of MMP-13 were greatest after helium pneumoperitoneum. After 5 days, collagen I/III ratio was significantly increased in the helium group. After 10 days, collagen I/III ratio was lowest in the CO(2) group. CONCLUSION: Our results suggest beneficial effects on systemic wound healing for helium pneumoperitoneum as compared to CO(2).
Authors: M Vaalamo; L Mattila; N Johansson; A L Kariniemi; M L Karjalainen-Lindsberg; V M Kähäri; U Saarialho-Kere Journal: J Invest Dermatol Date: 1997-07 Impact factor: 8.551
Authors: A Eljaafari; K Duperrier; S Mazet; C Bardin; J Bernaud; B Durand; L Gebuhrer; H Bétuel; D Rigal Journal: Hum Immunol Date: 1998-10 Impact factor: 2.850
Authors: E G Chekan; C Nataraj; E M Clary; T Z Hayward; F J Brody; J C Stamat; M C Fina; W S Eubanks; C J Westcott Journal: Surg Endosc Date: 1999-11 Impact factor: 4.584
Authors: E J Hanly; J M Fuentes; A R Aurora; S L Bachman; A De Maio; M R Marohn; M A Talamini Journal: Surg Endosc Date: 2006-07-24 Impact factor: 4.584