Literature DB >> 17217954

Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia).

Mariano Higes1, Pilar García-Palencia, Raquel Martín-Hernández, Aránzazu Meana.   

Abstract

In this report, an experimental infection of Apis mellifera by Nosema ceranae, a newly reported microsporidian in this host is described. Nosema free honeybees were inoculated with 125,000 N. ceranae spores, isolated from heavily infected bees. The parasite species was identified by amplification and sequencing the SSUrRNA gene of the administered spores. Three replicate cages of 20 honeybees each were prepared, along with one control cage (n=20) supplied with sugar syrup only. The infection rate was 100% at the dosage administered. The presence of Nosema inside ventricular cells was confirmed in the samples using ultrathin sectioning and transmission electron microscopy. By day 3 p.i. a few cells (4.4%+/-1.2) were observed to be parasitized, whereas by 6 days p.i. more than half of the counted cells (66.4%+/-6) showed different parasite stages, this value increasing on day 7 p.i. (81.5%+/-14.8). Only one control bee died on day 7 p.i. In the infected groups, mortality was not observed until day 6 p.i. (66.7%+/-5.6). Total mortality on day 7 p.i. was 94.1% in the three infected replicates and by day 8 p.i. no infected bee was alive. After the infection, the parasites invaded both the tip of folds and the basal cells of the epithelium and the autoinfective capacity of the spores seemed to spread the infection rapidly between epithelial cells. On day 3 p.i., mature spores could be seen inside host cell tissue implying that the developmental cycle had been completed. The large number of parasitized cells, even the regenerative ones, the presence of autoinfective spores and the high mortality rate demonstrate that N. ceranae is highly pathogenic to Apis mellifera. Possible relation with bee depopulation syndrome is discussed by authors.

Entities:  

Mesh:

Substances:

Year:  2007        PMID: 17217954     DOI: 10.1016/j.jip.2006.11.001

Source DB:  PubMed          Journal:  J Invertebr Pathol        ISSN: 0022-2011            Impact factor:   2.841


  108 in total

1.  Outcome of colonization of Apis mellifera by Nosema ceranae.

Authors:  Raquel Martín-Hernández; Aránzazu Meana; Lourdes Prieto; Amparo Martínez Salvador; Encarna Garrido-Bailón; Mariano Higes
Journal:  Appl Environ Microbiol       Date:  2007-08-03       Impact factor: 4.792

2.  Changes in the Bacteriome of Honey Bees Associated with the Parasite Varroa destructor, and Pathogens Nosema and Lotmaria passim.

Authors:  Jan Hubert; Martina Bicianova; Ondrej Ledvinka; Martin Kamler; Philip J Lester; Marta Nesvorna; Jan Kopecky; Tomas Erban
Journal:  Microb Ecol       Date:  2016-10-11       Impact factor: 4.552

3.  Effective gene silencing in a microsporidian parasite associated with honeybee (Apis mellifera) colony declines.

Authors:  Nitzan Paldi; Eitan Glick; Maayan Oliva; Yaron Zilberberg; Lucie Aubin; Jeffery Pettis; Yanping Chen; Jay D Evans
Journal:  Appl Environ Microbiol       Date:  2010-07-09       Impact factor: 4.792

4.  Effect of temperature on the biotic potential of honeybee microsporidia.

Authors:  Raquel Martín-Hernández; Aránzazu Meana; Pilar García-Palencia; Pilar Marín; Cristina Botías; Encarna Garrido-Bailón; Laura Barrios; Mariano Higes
Journal:  Appl Environ Microbiol       Date:  2009-02-20       Impact factor: 4.792

5.  Flow cytometry analysis of Nosema species to assess spore viability and longevity.

Authors:  J G Sánchez Collado; M Higes; L Barrio; R Martín-Hernández
Journal:  Parasitol Res       Date:  2014-02-20       Impact factor: 2.289

6.  Nosema spp. infections cause no energetic stress in tolerant honeybees.

Authors:  Christoph Kurze; Christopher Mayack; Frank Hirche; Gabriele I Stangl; Yves Le Conte; Per Kryger; Robin F A Moritz
Journal:  Parasitol Res       Date:  2016-03-15       Impact factor: 2.289

7.  Morphological, molecular, and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera.

Authors:  Yanping P Chen; Jay D Evans; Charles Murphy; Robin Gutell; Michael Zuker; Dawn Gundensen-Rindal; Jeff S Pettis
Journal:  J Eukaryot Microbiol       Date:  2009 Mar-Apr       Impact factor: 3.346

8.  Silencing the Honey Bee (Apis mellifera) Naked Cuticle Gene (nkd) Improves Host Immune Function and Reduces Nosema ceranae Infections.

Authors:  Wenfeng Li; Jay D Evans; Qiang Huang; Cristina Rodríguez-García; Jie Liu; Michele Hamilton; Christina M Grozinger; Thomas C Webster; Songkun Su; Yan Ping Chen
Journal:  Appl Environ Microbiol       Date:  2016-10-27       Impact factor: 4.792

9.  Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.

Authors:  R Scott Cornman; Yan Ping Chen; Michael C Schatz; Craig Street; Yan Zhao; Brian Desany; Michael Egholm; Stephen Hutchison; Jeffery S Pettis; W Ian Lipkin; Jay D Evans
Journal:  PLoS Pathog       Date:  2009-06-05       Impact factor: 6.823

10.  Interactions between Nosema microspores and a neonicotinoid weaken honeybees (Apis mellifera).

Authors:  Cédric Alaux; Jean-Luc Brunet; Claudia Dussaubat; Fanny Mondet; Sylvie Tchamitchan; Marianne Cousin; Julien Brillard; Aurelie Baldy; Luc P Belzunces; Yves Le Conte
Journal:  Environ Microbiol       Date:  2009-12-27       Impact factor: 5.491

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.