| Literature DB >> 17214965 |
Kisu Kim1, Youngmi Hur, En-Kyung Ryu, Jung-Hyo Rhim, Cha Yong Choi, Cheol-Min Baek, Jae-Ho Lee, Junho Chung.
Abstract
A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF-cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF-cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF-cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.Entities:
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Year: 2006 PMID： 17214965 DOI： 10.1016/j.bbrc.2006.12.164
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575