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Literature DB >> 17212457

Nanoscale cell adhesion ligand presentation regulates nonviral gene delivery and expression.

Hyun Joon Kong¹, Susan Hsiong, David J Mooney.

Abstract

It is hypothesized that the nanoscale organization of cell adhesion ligands in a synthetic ECM regulates nonviral gene delivery. This hypothesis was examined with pre-osteoblasts cultured on substrates which present varied density and spacing of synthetic adhesion ligands. The levels of gene transfer and expression were increased with the density of adhesion ligands, but decreased with the spacing of ligands, due to changes in the cell growth rate. This study provides a material-based control point on the nanometer scale for nonviral gene based therapies.

Entities: Gene

Mesh：

Substances：
Ligands

Year: 2007 PMID： 17212457 PMCID： PMC2581921 DOI： 10.1021/nl062485g

Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189

24 in total

Nanoscale cell adhesion ligand presentation regulates nonviral gene delivery and expression.

Review 1. RGD modified polymers: biomaterials for stimulated cell adhesion and beyond.

Review 2. Gene therapy for severe combined immunodeficiency.

Review 3. Tissue cells feel and respond to the stiffness of their substrate.

4. Non-viral gene delivery regulated by stiffness of cell adhesion substrates.

5. Multi-scale modeling to predict ligand presentation within RGD nanopatterned hydrogels.

Review 6. Artificial viruses: a nanotechnological approach to gene delivery.

7. Nanoscale Adhesion Ligand Organization Regulates Osteoblast Proliferation and Differentiation.

8. Surface-tethered DNA complexes for enhanced gene delivery.

9. Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation.

10. Alginate type and RGD density control myoblast phenotype.

1. Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds.

2. Differential uptake of DNA-poly(ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces.

3. Active scaffolds for on-demand drug and cell delivery.

Review 4. Polymers to direct cell fate by controlling the microenvironment.

5. Engineering clustered ligand binding into nonviral vectors: alphavbeta3 targeting as an example.

6. Optimizing siRNA efficacy through alteration in the target cell-adhesion substrate interaction.

7. Three-dimensional synthetic niche components to control germ cell proliferation.

Review 8. It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies.

Review 9. Combining topographical and genetic cues to promote neuronal fate specification in stem cells.

10. Network analysis of endogenous gene expression profiles after polyethyleneimine-mediated DNA delivery.