Literature DB >> 17209075

Quantitative assessment of the tetracycline resistance gene pool in cheese samples by real-time TaqMan PCR.

Michele Y Manuzon1, Scott E Hanna, Hongliang Luo, Zhongtang Yu, W James Harper, Hua H Wang.   

Abstract

A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.

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Year:  2007        PMID: 17209075      PMCID: PMC1828764          DOI: 10.1128/AEM.01994-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  6 in total

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2.  Development of a real-time PCR-based system targeting the 16S rRNA gene sequence for rapid detection of Alicyclobacillus spp. in juice products.

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4.  High-frequency conjugation system facilitates biofilm formation and pAMbeta1 transmission by Lactococcus lactis.

Authors:  Hongliang Luo; Kai Wan; Hua H Wang
Journal:  Appl Environ Microbiol       Date:  2005-06       Impact factor: 4.792

5.  The maintenance in the oral cavity of children of tetracycline-resistant bacteria and the genes encoding such resistance.

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6.  Improved extraction of PCR-quality community DNA from digesta and fecal samples.

Authors:  Zhongtang Yu; Mark Morrison
Journal:  Biotechniques       Date:  2004-05       Impact factor: 1.993

  6 in total
  8 in total

1.  Effective antibiotic resistance mitigation during cheese fermentation.

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6.  Characterization of antibiotic resistance in commensal bacteria from an aquaculture ecosystem.

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7.  Molecular identification and quantification of tetracycline and erythromycin resistance genes in Spanish and Italian retail cheeses.

Authors:  Ana Belén Flórez; Ángel Alegría; Franca Rossi; Susana Delgado; Giovanna E Felis; Sandra Torriani; Baltasar Mayo
Journal:  Biomed Res Int       Date:  2014-09-11       Impact factor: 3.411

8.  Antibiotic Administration Routes and Oral Exposure to Antibiotic Resistant Bacteria as Key Drivers for Gut Microbiota Disruption and Resistome in Poultry.

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  8 in total

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