Phylloplane proteins: emerging defenses at the aerial frontline?

The phylloplane, or leaf surface, is an interkingdom crossroads between plants and microorganisms, and secretion of antimicrobial biochemicals to aerial surfaces is thought to be one defensive strategy by which plants deter potential pathogens. Secondary metabolites on leaf surfaces are well documented but antimicrobial phylloplane proteins have only recently been identified. In this review, we describe the physical structures and biochemicals of the phylloplane and briefly discuss protein-based surface defenses of animals. We also review the emerging evidence pertaining to antimicrobial phylloplane proteins and mechanisms by which proteins can be released to the phylloplane, including biosynthesis (e.g. phylloplanins) by specific trichomes and delivery in guttation fluid from hydathodes. Future research should lead to exciting advances in our understanding of the phylloplane and to useful biotechnological interventions.