Literature DB >> 1720769

Digoxigenin as a probe label for in situ hybridization on skeletal tissues.

Y S Bland1, M A Critchlow, D E Ashhurst.   

Abstract

Non-specific staining was encountered using digoxigenin-labelled cDNA probes for in situ hybridization on sections of skeletal tissues. This staining was most pronounced in cartilaginous matrices. Experimental procedures indicate that the background staining is caused by antibody-binding to hydrophobic sites in the tissues revealed by proteolytic permeabilization. A protocol for minimizing this background is described.

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Year:  1991        PMID： 1720769     DOI： 10.1007/bf01042298

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  8 in total

1.  Changes in the distribution of fibrillar collagens in the collateral and cruciate ligaments of the rabbit knee joint during fetal and postnatal development.

Authors:  Y S Bland; D E Ashhurst
Journal:  Histochem J       Date:  1996-05

2.  Gene expression of matrix metalloproteinase-1 (interstitial collagenase) and matrix metalloproteinase-3 (stromelysin-1) in basal cell carcinoma by in situ hybridization using chondroitin ABC lyase.

Authors:  R Tsukifuji; Y Sakai; A Hatamochi; H Shinkai
Journal:  Histochem J       Date:  1997-05

3.  Fetal and postnatal development of the patella, patellar tendon and suprapatella in the rabbit; changes in the distribution of the fibrillar collagens.

Authors:  Y S Bland; D E Ashhurst
Journal:  J Anat       Date:  1997-04       Impact factor: 2.610

4.  Changes in the content of the fibrillar collagens and the expression of their mRNAs in the menisci of the rabbit knee joint during development and ageing.

Authors:  Y S Bland; D E Ashhurst
Journal:  Histochem J       Date:  1996-04

5.  The hip joint: the fibrillar collagens associated with development and ageing in the rabbit.

Authors:  Y S Bland; D E Ashhurst
Journal:  J Anat       Date:  2001-01       Impact factor: 2.610

6.  Development and ageing of the articular cartilage of the rabbit knee joint: distribution of the fibrillar collagens.

Authors:  Y S Bland; D E Ashhurst
Journal:  Anat Embryol (Berl)       Date:  1996-12

7.  The expression of collagen mRNAs in normally developing neonatal rabbit long bones and after treatment of neonatal and adult rabbit tibiae with transforming growth factor-beta 2.

Authors:  M A Critchlow; Y S Bland; D E Ashhurst
Journal:  Histochem J       Date:  1995-07

8.  Constitutive expression and modulation of the functional thrombin receptor in the human kidney.

Authors:  Y Xu; U Zacharias; M N Peraldi; C J He; C Lu; J D Sraer; L F Brass; E Rondeau
Journal:  Am J Pathol       Date:  1995-01       Impact factor: 4.307
  8 in total

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