Literature DB >> 1720768

Improved fixation of frozen lympho-haemopoietic tissue sections with hexazotized pararosaniline.

J P De Jong1, J S Voerman, P J Leenen, A J Van der Sluijs-Gelling, R E Ploemacher.   

Abstract

In this study, a simple single-step fixation method for frozen tissue sections is introduced using the hexazotized salt of Pararosaniline as preservative agent. Tissue preservation by this method was shown to be superior to the commonly-used fixation with acetone. Fixation with hexazotized Pararosaniline caused a minimal loss of antigenicity as demonstrated using twenty-three monoclonal antibodies directed against lympho-haemopoietic and stromal cells.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1720768     DOI： 10.1007/bf01042295

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  29 in total

1.  Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.

Authors:  M K Ho; T A Springer
Journal:  J Biol Chem       Date:  1983-01-10       Impact factor: 5.157

2.  Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200.

Authors:  W van Ewijk; P L van Soest; G J van den Engh
Journal:  J Immunol       Date:  1981-12       Impact factor: 5.422

3.  Tissue preparation for the demonstration of surface antigen by immunoperoxidase techniques.

Authors:  M A Judd; K J Britten
Journal:  Histochem J       Date:  1982-09

4.  A cell-surface molecule involved in organ-specific homing of lymphocytes.

Authors:  W M Gallatin; I L Weissman; E C Butcher
Journal:  Nature       Date:  1983 Jul 7-13       Impact factor: 49.962

5.  A scanning inverted microfluorometer with electronic shutter control for automatic measurements in micro-test plates.

Authors:  J E de Josselin de Jong; J F Jongkind; H R Ywema
Journal:  Anal Biochem       Date:  1980-02       Impact factor: 3.365

6.  Thy-1 determinants are present on many murine hematopoietic cells other than T cells.

Authors:  R S Basch; J W Berman
Journal:  Eur J Immunol       Date:  1982-05       Impact factor: 5.532

7.  Immunoelectron microscopy of cell surface antigens: a quantitative analysis of antibody binding after different fixation protocols.

Authors:  W Van Ewijk; R C Coffman; I L Weissman
Journal:  Histochem J       Date:  1980-05

8.  Single-cell immuno-beta-galactosidase staining of heterogeneous populations. Practical application on limited cell numbers.

Authors:  P J Leenen; M L Melis; W Van Ewijk
Journal:  Histochem J       Date:  1987-09

9.  A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among anti-Ia cytolytic T cell clones.

Authors:  A Pierres; P Naquet; A Van Agthoven; F Bekkhoucha; F Denizot; Z Mishal; A M Schmitt-Verhulst; M Pierres
Journal:  J Immunol       Date:  1984-06       Impact factor: 5.422

10.  Expression of the macrophage-specific antigen F4/80 during differentiation of mouse bone marrow cells in culture.

Authors:  S Hirsch; J M Austyn; S Gordon
Journal:  J Exp Med       Date:  1981-09-01       Impact factor: 14.307
View more
  5 in total

1.  Severe B cell deficiency and disrupted splenic architecture in transgenic mice expressing the E41K mutated form of Bruton's tyrosine kinase.

Authors:  G M Dingjan; A Maas; M C Nawijn; L Smit; J S Voerman; F Grosveld; R W Hendriks
Journal:  EMBO J       Date:  1998-09-15       Impact factor: 11.598

2.  A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.

Authors:  J P de Jong; J S Voerman; A J van der Sluijs-Gelling; R Willemsen; R E Ploemacher
Journal:  Cell Tissue Res       Date:  1994-03       Impact factor: 5.249

3.  Osteoclast development in the coculture system of periostless metatarsal bones and hemopoietic cells studied by in situ hybridization with a probe for Y chromosomes.

Authors:  C E Hagenaars; E W Kawilarang-de Haas; J Hazekamp; J Wiegant; P J Nijweide
Journal:  Calcif Tissue Int       Date:  1994-02       Impact factor: 4.333

4.  Thymic dendritic cells are primary targets for the oncogenic virus SL3-3.

Authors:  C H Uittenbogaart; W Law; P J Leenen; G Bristol; W van Ewijk; E F Hays
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103

5.  The Effects of Drying Techniques on Phytochemical Contents and Biological Activities on Selected Bamboo Leaves.

Authors:  Mohammad Amil Zulhilmi Benjamin; Shean Yeaw Ng; Fiffy Hanisdah Saikim; Nor Azizun Rusdi
Journal:  Molecules       Date:  2022-09-30       Impact factor: 4.927
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.