Literature DB >> 17207557

Tracking of human Y receptors in living cells--a fluorescence approach.

Ilka Böhme1, Karin Mörl, Darja Bamming, Cindy Meyer, Annette G Beck-Sickinger.   

Abstract

Non-invasive methods for studying biological processes in living cells have become very important, also in the field of GPCR biochemistry. Great advancements in the application of fluorescence techniques as well as in the development and improvement of novel fluorophores allow the visualization of dynamic processes. Using these technologies, problems concerning receptor biosynthesis, internalization, recycling and degradation can be investigated. Here we compare the application of the different fluorescent tags EYFP, Lumiotrade mark and SNAPtrade mark to track hY(1) and hY(5) receptors in living cells.

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Year:  2007        PMID： 17207557     DOI： 10.1016/j.peptides.2006.08.042

Source DB:  PubMed          Journal:  Peptides        ISSN： 0196-9781            Impact factor:   3.750


  8 in total

1.  Ligand-induced internalization and recycling of the human neuropeptide Y2 receptor is regulated by its carboxyl-terminal tail.

Authors:  Cornelia Walther; Stefanie Nagel; Luis E Gimenez; Karin Mörl; Vsevolod V Gurevich; Annette G Beck-Sickinger
Journal:  J Biol Chem       Date:  2010-10-18       Impact factor: 5.157

2.  Ligand-mimicking receptor variant discloses binding and activation mode of prolactin-releasing peptide.

Authors:  Daniel Rathmann; Diana Lindner; Stephanie H DeLuca; Kristian W Kaufmann; Jens Meiler; Annette G Beck-Sickinger
Journal:  J Biol Chem       Date:  2012-07-09       Impact factor: 5.157

3.  Oligomerization of neuropeptide Y (NPY) Y2 receptors in CHO cells depends on functional pertussis toxin-sensitive G-proteins.

Authors:  S L Parker; M S Parker; F R Sallee; A Balasubramaniam
Journal:  Regul Pept       Date:  2007-06-21

4.  Fluorescent labeling of tetracysteine-tagged proteins in intact cells.

Authors:  Carsten Hoffmann; Guido Gaietta; Alexander Zürn; Stephen R Adams; Sonia Terrillon; Mark H Ellisman; Roger Y Tsien; Martin J Lohse
Journal:  Nat Protoc       Date:  2010-09-23       Impact factor: 13.491

5.  The activity of prolactin releasing peptide correlates with its helicity.

Authors:  Stephanie H Deluca; Daniel Rathmann; Annette G Beck-Sickinger; Jens Meiler
Journal:  Biopolymers       Date:  2013-05       Impact factor: 2.505

6.  Importance of a N-terminal aspartate in the internalization of the neuropeptide Y Y2 receptor.

Authors:  Steven L Parker; Michael S Parker; Ying Y Wong; Renu Sah; Ambikaipakan Balasubramaniam; Floyd Sallee
Journal:  Eur J Pharmacol       Date:  2008-07-30       Impact factor: 4.432

7.  Illuminating the life of GPCRs.

Authors:  Ilka Böhme; Annette G Beck-Sickinger
Journal:  Cell Commun Signal       Date:  2009-07-14       Impact factor: 5.712

Review 8.  Simultaneous detection of the subcellular localization of RNAs and proteins in cultured cells by combined multicolor RNA-FISH and IF.

Authors:  Cindy Meyer; Aitor Garzia; Thomas Tuschl
Journal:  Methods       Date:  2016-09-21       Impact factor: 3.608
  8 in total

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