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Literature DB >> 172070

A sensitive radiochemical assay for angiotensin-converting enzyme (kinase II).

Abstract

[[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells.

Entities: Chemical Gene

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Year: 1975 PMID： 172070 PMCID： PMC1165618 DOI： 10.1042/bj1490297

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

10 in total

1. Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors: W M HUNTER; F C GREENWOOD
Journal: Nature Date: 1962-05-05 Impact factor: 49.962

2. NEW PROCEDURES FOR MEASUREMENT OF HUMAN PLASMA ANGIOTENSIN AND RENIN ACTIVITY LEVELS.

Authors: R BOUCHER; R VEYRAT; J DE CHAMPLAIN; J GENEST
Journal: Can Med Assoc J Date: 1964-01-25 Impact factor: 8.262

3. Direct spectrophotometric measurement of the peptide bond; application to the determination of acylase I.

Authors: M A MITZ; R J SCHLUETER
Journal: Biochim Biophys Acta Date: 1958-01

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Hydrolysis of bradykinin by angiotensin-converting enzyme.

Authors: F E Dorer; J R Kahn; K E Lentz; M Levine; L T Skeggs
Journal: Circ Res Date: 1974-06 Impact factor: 17.367

6. Purification and properties of angiotensin-converting enzyme from hog lung.

Authors: F E Dorer; J R Kahn; K E Lentz; M Levine; L T Skeggs
Journal: Circ Res Date: 1972-09 Impact factor: 17.367

7. Metabolism of [8-L-[14C] phenylalanine]-angiotensin I in the pulmonary circulation.

Authors: J W Ryan; R S Niemeyer; D W Goodwin; U Smith
Journal: Biochem J Date: 1971-12 Impact factor: 3.857

8. Hydrolysis of bradykinin and its higher homologues by angiotensin-converting enzyme.

Authors: F Dorer; J W Ryan; J M Stewart
Journal: Biochem J Date: 1974-09 Impact factor: 3.857

9. Replication of human endothelial cells in culture.

Authors: L J Lewis; J C Hoak; R D Maca; G L Fry
Journal: Science Date: 1973-08-03 Impact factor: 47.728

Review 10. Sequence determination.

Authors: P Edman
Journal: Mol Biol Biochem Biophys Date: 1970