Literature DB >> 172065

Radio-enzymic assay of oxo steroids by their reduction with the tritiated form of reduced nicotinamide--adenine dinucleotide.

J K Norymberski, N Stoddart, J B Wolstenholme.   

Abstract

A method of analysis is proposed based on the enzyme-catalysed transfer of tritium from [3H]NADH to suitable substrates. Its practicability is demonstrated on the examples of oestrone and progesterone with the respective use of the 3 beta, 17 beta- and 3 alpha, 20 beta-hydroxysteroid oxidoreductase. Specificity is tested by application to the analysis of the plasma of pregnant women and measurement of the 3H/14C ratios on purification of the enzymic reduction products.

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Year:  1975        PMID： 172065      PMCID： PMC1165185          DOI： 10.1042/bj1450047

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  The enzymatic transfer of hydrogen. IV. The reaction catalyzed by a beta-hydroxysteroid dehydrogenase.

Authors:  P TALALAY; F A LOEWUS; B VENNESLAND
Journal:  J Biol Chem       Date:  1955-02       Impact factor: 5.157

2.  The enzymatic transfer of hydrogen. III. The reaction catalyzed by malic dehydrogenase.

Authors:  F A LOEWUS; T T TCHEN; B VENNESLAND
Journal:  J Biol Chem       Date:  1955-02       Impact factor: 5.157

3.  [20 beta-Hydroxysteroid dehydrogenase, a new crystalline enzyme].

Authors:  H J HUEBENER; F G SAHRHOLZ; J SCHMIDT-THOME; G NESEMANN; R JUNK
Journal:  Biochim Biophys Acta       Date:  1959-09

4.  Stereospecificity of hydrogen transfer by pyridine nucleotide-linked hydroxysteroid dehydrogenases.

Authors:  J JARABAK; P TALALAY
Journal:  J Biol Chem       Date:  1960-07       Impact factor: 5.157

5.  [20 beta-Hydroxysteroid dehydrogenase. 2. Preparation and crystallization].

Authors:  H J HUEBENER; F G SAHRHOLZ
Journal:  Biochem Z       Date:  1960

6.  Steric, chiral and conformational aspects of the 3-hydroxy- and 20-hydroxysteroid dehydrogenase activities of cortisone reductase preparations.

Authors:  W Gibb; J Jeffery
Journal:  Biochim Biophys Acta       Date:  1972-04-07

7.  Stereochemistry of hydrogen transfer from pyridine nucleotides catalyzed by delta-4-3-oxosteroid 5-beta-reductase and 3-alpha-hydroxysteroid dehydrogenase from rat liver.

Authors:  I Björkhem; H Danielsson
Journal:  Eur J Biochem       Date:  1970-01

8.  Reaction mechanism and stereospecificity of 20 beta-hydroxysteroid dehydrogenase.

Authors:  G Betz; J C Warren
Journal:  Arch Biochem Biophys       Date:  1968-12       Impact factor: 4.013

9.  3 alpha-hydroxysteroid: NAD oxidoreductase activity in crystalline preparations of 20 beta-hydroxysteroid: NAD oxidoreductase.

Authors:  T Pocklington; J Jeffery
Journal:  Eur J Biochem       Date:  1968-12

10.  3-Hydroxy steroid dehydrogenase activities of cortisone reductase.

Authors:  W Gibb; J Jeffery
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857
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