Literature DB >> 17206375

Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare).

Suhong Li1, Tuoping Li, Wook-Dong Kim, Motomitsu Kitaoka, Shigeki Yoshida, Mitsutoshi Nakajima, Hideyuki Kobayashi.   

Abstract

The putative raffinose synthase gene from rice was cloned and expressed in Escherichia coli. The enzyme displayed an optimum activity at 45 degrees C and pH 7.0, and a sulfhydryl group was required for its activity. The enzyme was specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors, and sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose were recognized as galactosyl acceptors.

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Year:  2007        PMID： 17206375     DOI： 10.1007/s10529-006-9268-3

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  7 in total

1.  Raffinose synthase enhances drought tolerance through raffinose synthesis or galactinol hydrolysis in maize and Arabidopsis plants.

Authors:  Tao Li; Yumin Zhang; Ying Liu; Xudong Li; Guanglong Hao; Qinghui Han; Lynnette M A Dirk; A Bruce Downie; Yong-Ling Ruan; Jianmin Wang; Guoying Wang; Tianyong Zhao
Journal:  J Biol Chem       Date:  2020-05-04       Impact factor: 5.157

2.  Effects of chilling stress on the accumulation of soluble sugars and their key enzymes in Jatropha curcas seedlings.

Authors:  Haibo Wang; Ming Gong; Hu Xin; Lizhou Tang; Dongqin Dai; Yong Gao; Chao Liu
Journal:  Physiol Mol Biol Plants       Date:  2018-06-30

3.  Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390).

Authors:  Aurélie Egert; Felix Keller; Shaun Peters
Journal:  BMC Plant Biol       Date:  2013-12-20       Impact factor: 4.215

4.  Genome-Wide Expression Profiling Analysis of Kiwifruit GolS and RFS Genes and Identification of AcRFS4 Function in Raffinose Accumulation.

Authors:  Jun Yang; Chengcheng Ling; Yunyan Liu; Huamin Zhang; Quaid Hussain; Shiheng Lyu; Songhu Wang; Yongsheng Liu
Journal:  Int J Mol Sci       Date:  2022-08-09       Impact factor: 6.208

5.  Raffinose Family Oligosaccharides Act As Galactose Stores in Seeds and Are Required for Rapid Germination of Arabidopsis in the Dark.

Authors:  Roman Gangl; Raimund Tenhaken
Journal:  Front Plant Sci       Date:  2016-07-26       Impact factor: 5.753

6.  The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides.

Authors:  Xiaoming Sun; José Tomás Matus; Darren Chern Jan Wong; Zemin Wang; Fengmei Chai; Langlang Zhang; Ting Fang; Li Zhao; Yi Wang; Yuepeng Han; Qingfeng Wang; Shaohua Li; Zhenchang Liang; Haiping Xin
Journal:  J Exp Bot       Date:  2018-03-24       Impact factor: 6.992

7.  γ-Aminobutyric Acid Enhances Heat Tolerance Associated with the Change of Proteomic Profiling in Creeping Bentgrass.

Authors:  Zhou Li; Weihang Zeng; Bizhen Cheng; Ting Huang; Yan Peng; Xinquan Zhang
Journal:  Molecules       Date:  2020-09-18       Impact factor: 4.411
  7 in total

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